Global transcriptome analysis of mammary tissue during fetal bud formation and growth in two pig breeds
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ABSTRACT: The mammary gland development in swine begins at embryogenesis. The number and identity of relevant genes are unknown. Simultaneously, 24,123 probe-sets were screened, of which some 12,000 had present calls and were analysed for differential expression between different breeds (german landrace and pietrain) at different stages of development ( 63 and 91 dpc)
Project description:The mammary gland development in swine begins at embryogenesis. The number and identity of relevant genes are unknown. Simultaneously, 24,123 probe-sets were screened, of which some 12,000 had present calls and were analysed for differential expression between different breeds (german landrace and pietrain) at different stages of development ( 63 and 91 dpc) RNAs of teat from german landrace and pietrain at 63 and 91 dpc were hybridized to Affymetrix Porcine Genome Arrays.
Project description:The skeletal muscle development in swine begins at embryogenesis. Simultaneously, 24,123 probe-sets were screened, analysed for differential expression between different breeds (german landrace and pietrain) at different stages of development (35, 63, 91 dpc and adult stage). RNAs of skeletal muscle from german landrace and pietrain at 35, 63, 91 dpc and adult stages were hybridized to Affymetrix GeneChip Porcine Genome Array.
Project description:The skeletal muscle development in swine begins at embryogenesis. Simultaneously, 24,123 probe-sets were screened, analysed for differential expression between different breeds (german landrace and pietrain) at different stages of development (35, 63, 91 dpc and adult stage).
Project description:Total of 675 porcine miRNA candidates datasets revealed by 100% aligment homologs and review literatures publication. Probe-sets were screened, analysed for differential expression between different breeds (german landrace and pietrain) at different stages of development (63, 91 dpc and adult stage) microRNAs of skeletal muscle from german landrace and pietrain at 63, 91 dpc and adult stages were hybridized to Affymetrix GeneChip miRNA arrays.
Project description:Total of 675 porcine miRNA candidates datasets revealed by 100% aligment homologs and review literatures publication. Probe-sets were screened, analysed for differential expression between different breeds (german landrace and pietrain) at different stages of development (63, 91 dpc and adult stage)
Project description:German landrace gilts were fed a low protein diet (LP, 6% CP) throughout their whole pregnancy. Subsequently hepatic transcriptome profiles of the offspring were analysed at prenatal (94 dpc) and postnatal stages (1, 28, 188 dpn)
Project description:German landrace gilts were fed a high protein diet (HP, 30% CP) throughout their whole pregnancy. Subsequently muscle transcriptome profiles of the offspring were analysed at prenatal (94 dpc) and postnatal stages (1, 28, 188 dpn)
Project description:German landrace gilts were fed an adequate protein diet (AP, 12% CP) throughout their whole pregnancy. Subsequently muscle transcriptome profiles of the offspring were analysed at prenatal (94 dpc) and postnatal stages (1, 28, 188 dpn)
Project description:German landrace gilts were fed a low protein diet (LP, 6% CP) throughout their whole pregnancy. Subsequently muscle transcriptome profiles of the offspring were analysed at prenatal (94 dpc) and postnatal stages (1, 28, 188 dpn)
Project description:German landrace gilts were fed a high protein diet (HP, 30% CP) throughout their whole pregnancy. Subsequently hepatic transcriptome profiles of the offspring were analysed at prenatal (94 dpc) and postnatal stages (1, 28, 188 dpn) Keywords: ANOVA