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Tomato ripe fruits:Lycb-1 transgenic line vs Wild type

ABSTRACT: The enzyme lycopene β-cyclase (LYCB) is responsible for the synthesis of β-carotene, a valuable component of the human diet. To understand the effect of the high β-Carotene content accumulation on plants carotenoid biosynthesis and global genes expression，a tomato engineered to constitutively express Lycb-1 accumulated a high level of β-carotene was used in this reasearch.Microarray analysis in the ripe stage revealed that the constitutive expression of Lycb-1 differentially regulated a number of genes involved in the synthesis of fatty acids, flavones, flavonols, flavonoids and phenylpropanoids, in the degradation of limonene and pinene, in starch and sucrose metabolism and in photosynthesis.

ORGANISM(S): Solanum lycopersicum

PROVIDER: GSE33435 | GEO | 2012/12/12

SECONDARY ACCESSION(S): PRJNA148687

REPOSITORIES: GEO

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Project description:Tomato fruit ripening is under the control of ethylene as well as a group of ethylene-independent transcription factors, including NON-RIPENING (NOR) and RIPENING INHIBITOR (RIN). During ripening, the linear carotene lycopene accumulates at the expense of cyclic carotenoids. Fruit-specific overexpression of LYCOPENE β-CYCLASE (LCYb) under the control of the PHYTOENE DESATURASE (PDS) promoter resulted in increased levels of β-carotene and ABA and in decreased ethylene levels. Genes regulated by ABA, or involved in its synthesis and signaling, were overexpressed, while those associated with ethylene and cell wall remodeling were repressed. In agreement with the transcriptional data, LCYb-overexpressing fruits exhibited increased density of cell wall material containing linear, under-methylated pectins and displayed an array of additional ripening phenotypes, including delayed softening, increased turgor, enhanced shelf life and a thicker cuticle with a higher content of cutin monomers and triterpenoids. The levels of several primary metabolites and phenylpropanoids also changed in the transgenics, which could be attributed to delayed fruit ripening and to ABA respectively. Network correlation analysis suggests that ABA, acting through NOR and RIN, is responsible for many of the above phenotypes. These data reinforce suggestions that ABA plays an important role in tomato fruit ripening and provide clues that fruit b-carotene, acting as a precursor for ABA, actively participates in controlling the ripening process rather than merely being an output thereof. Overexpression of a LCYb gene from Arabidopsis under the control of the ripening-associated PDS promoter leads to ripe tomato fruits accumulating high β-carotene levels. Using several independent transgenic lines, we conducted a system-wide study of the effect of increased β-carotene levels on tomato fruit ripening and shelf life. Our data suggest that β-carotene, acting through ABA, is involved in a regulatory loop within the network controlling tomato fruit ripening.

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Tomato ripe fruits:Lycb-1 transgenic line vs Wild type

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