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Expression data obtained from HPBALL cell line

ABSTRACT: The experiment was designed in order to knock down the expression of TLX3 gene in T-ALL cell line We used microarrays to identify the gene expression signatures associated with TLX3 knock down in HPBALL

ORGANISM(S): Homo sapiens

PROVIDER: GSE33540 | GEO | 2011/11/08

SECONDARY ACCESSION(S): PRJNA154269

REPOSITORIES: GEO

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Expression data obtained from HPBALL cell line

Project description:The experiment was designed in order to knock down the expression of TLX3 gene in T-ALL cell line We used microarrays to identify the gene expression signatures associated with TLX3 knock down in HPBALL HPBALL cell lines were infected with pLKO.1-shTLX3 and pLKO.1-luc control lentivirus. After 5 days of puromycin selection cells were collected and RNA was extracted in order to analyze the global changes in gene expression by Affymetrix U133 Plus 2 array.

2011-11-08 | E-GEOD-33540 | biostudies-arrayexpress

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Expression data obtained from ALLSIL cell line

Project description:The experiment was designed in order to knock down the expression of TLX1 gene in T-ALL cell line We used microarrays to identify the gene expression signatures associated with TLX1 knock down in ALLSIL ALLSIL cell lines were infected with pLKO.1-shTLX1 and pLKO.1-luc control lentivirus. After 5 days of puromycin selection cells were collected and RNA was extracted in order to analyze the global changes in gene expression by Affymetrix U133 Plus 2 array.

2011-11-08 | E-GEOD-33539 | biostudies-arrayexpress

Expression data from knock-down ES stable cell line

Project description:In order to identify the effects of the knock-down of the gene of interest on the mouse ES transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the knock-down cell line. Transcriptome analysis of the knock-down transgenic mouse ES cell line. The knock-down cell line (shE13) was generated by stably expressing a specific short-hairpin RNA against E13 sequence thus knocking-down E13 expression in parental mouse ES cell line E14Tg2a.4 (E14, Hooper M et al., 1987). The specific mouse gene knocked down in the ES cell line is E130012A19Rik.

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RHOQ knock-down Expression data from colorectal cancer cell line HCT-116

Project description:We used microarrays to detail the global gene expression in cell line with or without RHOQ knock-down.

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Expression data from knock-down ES stable cell line

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TYRP1 knock-down in human melanoma cell line (501Mel)

Project description:Efficient TYRP1 knock-down (KD) have been performed in human melanoma cell line expressing a high level of TYRP1 mRNA and protein in order to identify the molecular pathway explaining the decrease of cell proliferation in TYRP1 KD cells.

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synthetic construct

Project description:Direct calibration of PICKY-designed microarrays

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Expression data from glp-1(e2141) hermaphrodites maintained with or without males for 8 days

Project description:Males induce dramatic physiological changes to hermaphrodites, including a significant shortening of lifespan. We have termed this effect as male-induced demise (MID) of hermaphrodites. This experiment was performed to analyse changes in gene expression due to the presence of males. We have shown that Knock down of utx-1 ameliorates the MID. In this experiment we also examine male-induce gene expression that may be altered when knocking down expression of utx-1 via RNAi. We used microarrays to detail the global program of gene expression andidentified distinct classes of up-regulated and down-regulated genes upon the addition of males.

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