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Gene expression response in roots of Medicago truncatula mutants treated with Myc LCOs

ABSTRACT: Legumes interact with soil fungi, leading to the development of arbuscular mycorrhizal (AM) roots. Diffusible AM fungal signals were identified as sulphated and non-sulphated LCOs (sMyc-LCOs and nsMyc-LCOs). Applying Myc-LCOs on roots of symbiotic mutants, we used GeneChips to detail the global programme of gene expression in these mutants in response to the external application of Myc-LCOs.

ORGANISM(S): Medicago sativa Medicago truncatula Sinorhizobium meliloti

PROVIDER: GSE33637 | GEO | 2012/05/31

SECONDARY ACCESSION(S): PRJNA154315

REPOSITORIES: GEO

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Gene expression response in roots of Medicago truncatula mutants treated with Myc-LCOs

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2012-05-30 | E-GEOD-33636 | biostudies-arrayexpress

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Project description:Legumes interact with rhizobia, leading to the development of root nodules. Diffusible rhizobial signals were identified as Nod-LCOs. Applying Nod-LCOs on plantlet roots, we used GeneChips to detail the global programme of gene expression in response to the external application of Nod-LCOs.

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Gene expression data from arbuscular mycorrhizal and phosphate-treated Medicago truncatula roots.

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Medicago truncatula

Project description:Gene expression response in roots of Medicago truncatula mutants treated with Myc-LCOs

| PRJNA279162 | ENA

Gene expression data from arbuscular mycorrhizal and phosphate-treated Medicago truncatula roots.

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Medicago truncatula Transcriptome or Gene expression

Project description:Our global transcriptomic approach to study Myc-LCO signalling in M. truncatula used an experimental design of three genotypes â A17 (WT), dmi3 and nsp1 â and three treatments â sulphated Myc-LCOs (S Myc-LCOs), nonsulphated Myc-LCOs (NS Myc-LCOs), and solvent controls (acetonitrile, ACN). We pooled two early treatment time points (2 + 4 h) based on preliminary data and reports of transcriptional changes occurring within hours of Myc-LCO treatment. Three biological replicates were sequenced per condition. We used M. truncatula dmi3 and nsp1 mutants, described originally as NodâMycâ and NodâMyc+, respectively, and the two major types of Myc-LCOs (sulphated and nonsulphated) to determine overlaps and specificities of Myc-LCO responses. Myc-LCOs were used at 10â6 M because they are biologically active at about a 100-fold higher concentration than Nod factors, which have been used at 10â7, 10â8 M in transcriptomic studies.

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