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Gene expression response in roots of Medicago truncatula mutants treated with Myc-LCOs

ABSTRACT: Legumes interact with soil fungi, leading to the development of arbuscular mycorrhizal (AM) roots. Diffusible AM fungal signals were identified as sulphated and non-sulphated LCOs (sMyc-LCOs and nsMyc-LCOs). Applying Myc-LCOs on roots of symbiotic mutants, we used GeneChips to detail the global programme of gene expression in these mutants in response to the external application of Myc-LCOs. Keywords: Expression profiling by array

ORGANISM(S): Medicago sativa Sinorhizobium meliloti Medicago truncatula

PROVIDER: GSE67166 | GEO | 2015/11/12

SECONDARY ACCESSION(S): PRJNA279162

REPOSITORIES: GEO

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Gene expression response in roots of Medicago truncatula mutants treated with Myc LCOs

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Project description:Legumes interact with soil fungi, leading to the development of arbuscular mycorrhizal (AM) roots. Diffusible AM fungal signals were identified as sulphated and non-sulphated LCOs (sMyc-LCOs and nsMyc-LCOs). Applying Myc-LCOs on roots of symbiotic mutants, we used GeneChips to detail the global programme of gene expression in these mutants in response to the external application of Myc-LCOs. To harvest tissues for transcriptome profiling, three biological replicates consisting of 10 plantlets per treatment were selected. After 6 h of incubation in the climate chamber, 10 plantlets per batch were removed from the treatment (Myc-LCOs) or control solutions and harvested. During harvest, one mm of the root tip of each plantlet was removed and discarded. The remaining 2 to 2.5 cm of the distal root region were cut off and directly frozen in liquid nitrogen.

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Medicago truncatula

Project description:Gene expression response in roots of Medicago truncatula mutants treated with Myc-LCOs

| PRJNA279162 | ENA

Gene expression data from arbuscular mycorrhizal and phosphate-treated Medicago truncatula roots.

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