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Role of miRNAs in the differentiation of human embryonic stem cells to an endothelial cell lineage

ABSTRACT: To assess changes in the miRNA-ome during the early stages of endothelial differentiation, the expression of mature human miRNAs at days 2, 4 and 10 of endothelial differentiation (vs pluripotent time-matched samples) in SA461 hESC cell line was analysed in a two-channel microarray. Expression of the pluripotency-associated miRNAs miR-302a – d and miR-372 and miR-373 was significantly suppressed with progression of differentiation. The expression of miR-99b, miR-181a and miR-181b was, in contrast, increased in a time- and differentiation-dependent manner compared to time-matched pluripotent samples. The expression profiles were validated and good concordance was observed between microarray, Q-PCR and Northern blot analysis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE33675 | GEO | 2012/01/01

SECONDARY ACCESSION(S): PRJNA148295

REPOSITORIES: GEO

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Role of miRNAs in the differentiation of human embryonic stem cells to an endothelial cell lineage

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