Methylation profiling

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Genome-wide CpG methylation during osteogenic and myogenic differentiation from adipose- derived stem cells.


ABSTRACT: In the current study, we have performed a high-throughput CpG methylation analysis of well characterized and defined populations of human adipose-derived stem cells (hASCs) before and after in vitro induction of osteogenic and myogenic differentiation that allows identifying DNA methylation- regulated differentiation genes. We have also address the extent of the epigenetic programming of hASCs- derived differentiated cells by comparing the methylation profiling of these cells with their somatic counterparts from primary tissues. Finally, we also compared the patterns of CpG methylation of hASCs (and their derivatives) with the methylation profiles of myosarcoma and osteosrcoma cell lines. All the CpG methylation studies have been performed with the Infinium 27K methylation arrays (from Illumina).

ORGANISM(S): Homo sapiens

PROVIDER: GSE33896 | GEO | 2014/01/02

SECONDARY ACCESSION(S): PRJNA148337

REPOSITORIES: GEO

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