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Identification of high temperature and daylength responsive genes in barley

ABSTRACT: High temperature influences plant development and can reduce crop yields. We used the Agilent Barley Gene Expression Microarray to identify high temperature responsive genes in cereals. In temperate cereals, such as wheat and barley, high temperature results in rapid progression through reproductive development in long-days but inhibits early stages of reproductive development in short-days. We examined the transcriptome of barley plants grown at two different temperatures, 15°C or 25°C, in either long or short-days. Under these conditions early reproductive development was accelerated by high temperature in long-days but inhibited by high temperature in short-days. To control for the effect of temperature on vegetative growth, plants were sampled at the same stage of vegetative development (leaf emergence) in all treatments. Transcriptome analysis identified genes that show changed transcript levels in response to daylength (long versus short-days), genes that show changed transcript levels in response to temperature (15°C versus 25°C), and small groups of genes that show changed transcript levels only in response to specific combinations of daylength and temperature. For example, genes that are upregulated only under long-days and high temperature: conditions in which early reproductive development proceeds rapidly. The temperature responsive genes identified here offer potential candidates for developmental regulators controlling the developmental response of cereal crops to high temperatures.

ORGANISM(S): Hordeum vulgare

PROVIDER: GSE33938 | GEO | 2012/04/04

SECONDARY ACCESSION(S): PRJNA150039

REPOSITORIES: GEO

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Identification of high temperature and daylength responsive genes in barley

Project description:High temperature influences plant development and can reduce crop yields. We used the Agilent Barley Gene Expression Microarray to identify high temperature responsive genes in cereals. In temperate cereals, such as wheat and barley, high temperature results in rapid progression through reproductive development in long-days but inhibits early stages of reproductive development in short-days. We examined the transcriptome of barley plants grown at two different temperatures, 15M-BM-0C or 25M-BM-0C, in either long or short-days. Under these conditions early reproductive development was accelerated by high temperature in long-days but inhibited by high temperature in short-days. To control for the effect of temperature on vegetative growth, plants were sampled at the same stage of vegetative development (leaf emergence) in all treatments. Transcriptome analysis identified genes that show changed transcript levels in response to daylength (long versus short-days), genes that show changed transcript levels in response to temperature (15M-BM-0C versus 25M-BM-0C), and small groups of genes that show changed transcript levels only in response to specific combinations of daylength and temperature. For example, genes that are upregulated only under long-days and high temperature: conditions in which early reproductive development proceeds rapidly. The temperature responsive genes identified here offer potential candidates for developmental regulators controlling the developmental response of cereal crops to high temperatures. Gene expression was assayed in vernalized barley plants grown at 15M-BM-0C or 25M-BM-0C in short or long-days until leaf 4 was visible on the main stem. Three replicate samples, consisting of 3 plants per sample, were assayed for each treatment.

2012-04-03 | E-GEOD-33938 | biostudies-arrayexpress

Early effects of the photoperiodic induction of flowering on the root transcriptome.

Project description:The induction of flowering by the photoperiodic pathway (i.e. an increase in daylength) involves the production of systemic signals that induce the transition to reproductive development. In order to evaluate the early events occurring in the roots during the photoperiodic induction of flowering, we harvested the roots of 7 week-old Col-0 Arabidopsis thaliana plants grown plants exposed to a single 22-hour long day. Control plants were maintained under 8-hour short days. Plants were grown in hydroponics and roots tissues were harvested 16 and 22 hours after the beginning of the light period.

2016-06-30 | E-MTAB-4130 | biostudies-arrayexpress

Hordeum vulgare

Project description:Identification of high temperature and daylength responsive genes in barley

| PRJNA150039 | ENA

Microarray analysis of Arabidopsis Col-0 and catalase 2 mutant define interactions between CO2, daylength and H2O2

Project description:Growth daylength, ambient CO2 level, and intracellular hydrogen peroxide (H2O2) availability all impact plant function by modulating signalling pathways, but interactions between them remain unclear. Using a whole-genome transcriptomics approach, we exploited the conditional photorespiratory nature of the catalase-deficient cat2 mutant to identify gene expression patterns responding to these three factors. Arabidopsis Col-0 and cat2 grown for 5 weeks in high CO2 in short days (SD) were transferred to air in SD or long days (LD), and microarray analysis was performed. Of more than 500 genes differentially expressed in Col-0 between high CO2 and transfer to air in SD, the response of about one-third was attenuated by transfer to air in LD. H2O2-responsive genes in cat2 were highly dependent on daylength. The majority of H2O2-induced genes were more strongly up-regulated after transfer to air in SD than to LD, while a smaller number showed an opposing pattern. Responses of other H2O2-dependent genes indicate redox-modulation of the daylength control of fundamental cell processes. The overall analysis provides evidence that (1) CO2 level modulates stress-associated gene expression; (2) both CO2 and H2O2 interact with daylength and photoreceptor signalling pathways; and (3) cellular signalling pathways may be primed to respond to increased H2O2 in a daylength-determined manner. Two genotypes x five conditions experiment, including Arabidopsis Col0WT and cat2-1 plants grown in soil for 5 weeks in a 8h light/16h dark (short day) regime at high CO2 concentration (3000 ppm CO2) and subsequently transferred to air (400 ppm CO2) in a short day or long day (16h light/8h dark) regime for 2 and 4 days. Three biological replicates were used that consist each of a pool of two leaves from different plants. Each sample was hybridized to one Genechip® Arabidopsis ATH1 Genome Array (Affymetrix).

2012-03-07 | E-GEOD-27985 | biostudies-arrayexpress

Transcription profiling of Populus x canescens developing and mature xylem under short-days and drought stress treatment

Project description:Populus x canescens were kept in climate chambers for a growing period of 8 weeks. Afterwards, treatment started for five weeks in total. Plant were first treated with different daylength (long-days or short-days) under well-watered conditions. After 2 weeks, half of each daylength treatment was drought stressed while the other half was still well-watered. After further three weeks, all poplars were harvested and samples of developing and mature xylem were taken and directly frozen in liquid nitrogen. These samples were taken for RNA extraction and following RNA-sequencing.

2018-10-21 | E-MTAB-7288 | biostudies-arrayexpress

Microarray analysis of Arabidopsis Col-0 and catalase 2 mutant define interactions between CO2, daylength and H2O2

2012-03-07 | GSE27985 | GEO

RNA-Seq of developing shoot apical meristems of barley cultivar Bonus and two allelic praematurum-c/mat-c mutants mat.c.907 (NGB 116862) and mat.c.943 (NGB 117474) grown under long- and short-day photoperiods

Project description:Natural variation in the barley homolog of CENTRORADIALIS (HvCEN), was found to contribute to the expansion of barley cultivation into diverse habitats. It has been shown that induced hvcen mutants, originally designated as praematurum-c/maturity-c (mat-c) mutants, flowered a few days earlier under natural long-day conditions. All hvcen mutants flowered early and showed a reduction in spikelet number per spike, tiller number and yield in the outdoor experiments. Further evaluating development of main shoot apex of hvcen mutants and wild type under controlled long day and short day conditions showed that mutations in hvcen accelerated spikelet initiation and reduced axillary bud number in a photoperiod independent manner, but promoted floret development only under long days. In this project we investigate the pleiotropic effects of HvCEN on developmental timing and shoot and spike morphologies of barley and dependence on these effects on photoperiod. This RNAseq dataset was generated to identify the putative transcriptional targets of HvCEN. To this end, we used global transcriptome profiling in developing shoot apices and inflorescences of two allelic hvcen mutants (mat-c.907 and mat-c.943) and wild-type (Bonus) plants grown under long- and short-day photoperiods.

2019-04-23 | E-MTAB-7807 | biostudies-arrayexpress

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Project description:High temperature stress, like any abiotic stress, impairs the physiology and development of plants, including the stages of seed setting and ripening. In this study we used the 22K Barley1 GeneChip microarray to investigate the response of developing barley (Hordeum vulgare) caryopses at 12 days post anthesis to 0.5h, 3h and 6h of heat stress exposure.

2010-11-22 | GSE23896 | GEO

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Project description:Young Drosophila females respond to low temperature and short photoperiod by developmental arrest of the ovaries. This form of reproductive diapause is a winter adaptation. Here we used Affymetrix microarrays to study global expression in female heads associated with long vs. short day.

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