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Identification of target genes of ABI3 [Agilent 44k]

ABSTRACT: ABI3 is a key regulator of seed development in Arabidopsis and other plants.To identify genes regulated by ABI3 we performed array based transcriptome analysis of Dexamethasone inducible ABI3 transgenic seedlings. ABI3 mostly in concert with abscisic acid (ABA) was found to activate genes specifically expressed during the maturation phase of seed development.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE33951 | GEO | 2012/06/01

SECONDARY ACCESSION(S): PRJNA154243

REPOSITORIES: GEO

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Identification of target genes of ABI3 [Agilent 44k]

Project description:ABI3 is a key regulator of seed development in Arabidopsis and other plants.To identify genes regulated by ABI3 we performed array based transcriptome analysis of Dexamethasone inducible ABI3 transgenic seedlings. ABI3 mostly in concert with abscisic acid (ABA) was found to activate genes specifically expressed during the maturation phase of seed development. Two weeks old wildtype and 35S::ABI3::GR seedlings were treated with ABA, Dexamethasone (DEX) and ABA plus Dexamethasone for 4 h. Two biological independent experiments were performed. Wildtype was induced to control for DEX-induction of genes.

2012-05-31 | E-GEOD-33951 | biostudies-arrayexpress

Identification of target genes of ABI3 [Agilent 2x244k]

Project description:ABI3 is a key regulator of seed development in Arabidopsis and other plants.To identify DNA fragments(promoters) bound by ABI3 we performed array based ChIP analysis.

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Identification of target genes of ABI3 [Agilent 2x244k]

Project description:ABI3 is a key regulator of seed development in Arabidopsis and other plants.To identify DNA fragments(promoters) bound by ABI3 we performed array based ChIP analysis. Two ChIP experiments were performed with chromatin of seeds of Arabidopsis and an anti-ABI3 antibody directed against a N-terminal fragment of ABI3, raised in rabbits. Non precipitated chromatin (input) served as control.

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Identification of target genes of ABI3 [SAP]

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Identification of target genes of ABI3 [SAP]

Project description:ABI3 is a key regulator of seed development in Arabidopsis and other plants.To identify DNA fragments (promoters) bound by ABI3 we performed array based ChIP analysis. Five ChIP-chip experiments were performed with chromatin of seeds of Arabidopsis and an anti-ABI3 antibody directed against a N-terminal fragment of ABI3, raised in rabbits. Non precipitated chromatin (input) served as control.

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ABI3 Network Walking

Project description:Deciphering gene regulatory networks (GRNs) is a key for understanding gene expression regulations in living systems. Here, we describe the investigation of the ABSCISIC ACID INSENSITIVE 3 (ABI3) plant transcription factor GRN vicinity by a technique called Network Walking. The method involves transient transformation of protoplasts and inducible nuclear re-localization of transcription factors along with transcriptomic analysis. This genome-wide approach allowed the de novo recovery of i) direct and indirect ABI3 target genes, ii) cis-binding site preference, and iii) biological processes regulated by this canonical abscisic acid response factor. This work improves our knowledge of ABI3 action by inferring network motifs (such as Feed Forwar Loops) under its influence. The novel high-throughput-oriented technique will help accelerate GRN systems investigations in plants, as well as in other organisms. This work studies ABI3 direct and indirect targets by a technique named Network Walking. Root/protoplasts were treated with or without dexamethasone (DEX) and cycloheximide (CHX). 3 reps each.

2013-01-01 | E-GEOD-33344 | biostudies-arrayexpress

ABI3 TARGET approach

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Direct and indirect targets of Arabidopsis ABI3: genome wide binding sites [gene expression]

Project description:ABSCISIC ACID INSENSITIVE3 (ABI3) is a transcription factor that is a member of the B3 domain family and is involved in seed development. To understand how it controls development, we mapped genome-wide binding sites by ChIP-chip and combined this data wi Ran Tian, Fangfang Wang, Qiaolin Zheng, Venus Niza, A. Bruce Downie and Sharyn E. Perry (2020)

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Direct and indirect targets of Arabidopsis ABI3: genome wide binding sites [ChIP-chip]

Project description:ABSCISIC ACID INSENSITIVE3 (ABI3) is a transcription factor that is a member of the B3 domain family and is involved in seed development. To understand how it controls development, we mapped genome-wide binding sites by ChIP-chip and combined this data with transcriptomic data obtained by microarrays. Ran Tian, Fangfang Wang, Qiaolin Zheng, Venus Niza, A. Bruce Downie and Sharyn E. Perry (2020)

2020-05-27 | GSE150559 | GEO

Comparison of genes differentially expressed between Medicago R108 and abi3 mutants (NF3185 and NF6003) during different stages of seed development (16, 24, 32 and 40 DAP)

Project description:ABI3 is a B3-domain transcription factor that acts as a master regulator of seed maturation. To identify genes that are regulated specifically by ABI3 in the model legume Medicago R108 during seed development, using the Medicago NimbleGen chip, a transcriptomic analysis was performed between abi3 mutants (NF3185 and NF6003) and R108 wild-type at 4 stages of seed development.

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