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Karyotype analysis of radicicol-resistant yeast colonies by array CGH (Radr1,2,3)

ABSTRACT: Aneuploidy, a state of having uneven numbers of chromosomes, is a form of large-effect mutation able to confer adaptive phenotypes under diverse stress conditions. Here we investigate whether pleiotropic stresses could in turn induce aneuploidy in budding yeast. Cells exposed to radicicol, an Hsp90 inhibitor, exhibit chromosomal instability. To assess the karyotype of cells with an acquired resistance to radicicol, array CGH was used to compare three stably resistant yeast colonies to a WT strain.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE34085 | GEO | 2011/12/31

SECONDARY ACCESSION(S): PRJNA156501

REPOSITORIES: GEO

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Karyotype analysis of radicicol-resistant yeast colonies by array CGH (Radr1,2,3)

Project description:Aneuploidy, a state of having uneven numbers of chromosomes, is a form of large-effect mutation able to confer adaptive phenotypes under diverse stress conditions. Here we investigate whether pleiotropic stresses could in turn induce aneuploidy in budding yeast. Cells exposed to radicicol, an Hsp90 inhibitor, exhibit chromosomal instability. To assess the karyotype of cells with an acquired resistance to radicicol, array CGH was used to compare three stably resistant yeast colonies to a WT strain. Single replicates of DNA prepared from radicicol-resistant yeast colonies are compared to a control WT colony.

2011-12-31 | E-GEOD-34085 | biostudies-arrayexpress

Genome structure of radicicol-induced aneuploids in yeast by array CGH

Project description:Aneuploidy, a state of having uneven numbers of chromosomes, is a form of large-effect mutation able to confer adaptive phenotypes under diverse stress conditions. Here we investigate whether pleiotropic stresses could in turn induce aneuploidy in budding yeast. Radicicol was shown to induce high level missegregation of an artificial chromosome (CF). This experiment examines possible aneuploidy of other (“natural”) chromosomes induced by radicicol. Red colonies (lost CF) were first screened by FACS. Possible aneuploids were then subjected to hybridization, with WT as the control.

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Genome structure of radicicol-induced aneuploids in yeast by array CGH

Project description:Aneuploidy, a state of having uneven numbers of chromosomes, is a form of large-effect mutation able to confer adaptive phenotypes under diverse stress conditions. Here we investigate whether pleiotropic stresses could in turn induce aneuploidy in budding yeast. Radicicol was shown to induce high level missegregation of an artificial chromosome (CF). This experiment examines possible aneuploidy of other (“natural”) chromosomes induced by radicicol.

2011-12-31 | GSE34086 | GEO

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Project description:This SuperSeries is composed of the following subset Series: GSE34085: Karyotype analysis of radicicol-resistant yeast colonies by array CGH (Radr1,2,3) GSE34086: Genome structure of radicicol-induced aneuploids in yeast by array CGH Refer to individual Series

2011-12-31 | E-GEOD-34087 | biostudies-arrayexpress

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Project description:Karyotype analysis of radicicol-resistant yeast colonies by array CGH (Radr1,2,3)

| PRJNA156501 | ENA

Transcription profiling of wild type and delta-sba1 yeast treated with radicicol to study gene regulation by sba1

Project description:We wanted to study regulation of gene regulation by sba1 (ortholog of mammalian p23) in yeast. sba1/p23 binds to Hsp90 and acts as a co-chaperone. We have a small molecule, radicicol, an analog of geldanamycin to inhibit the binding of p23 to Hsp90. Consequently, we have analyzed the gene expression profile in 4 conditions, wt, delta-sba1; and when Hsp90 function is impaired, that is, wt and delta-sba1 treated with radicicol.

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CGH analysis of mouse embryonic stem cells trisomic for chromosome 6, 8, 11,12 and 15

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