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Gene expression study after 3 days of cold exposure in a Solanum tuberosum cultivar called Desirée

ABSTRACT: The molecular response to cold exposure was studied in the leaves of an S. tuberosum clone using cDNA microarray. Differentially expressed genes were classified according to their known or predicted function and their expression ratio as compared to the control. The major changes upon cold exposure in Desirée leaves were observed in the photosynthesis-related genes, which were down-regulated, whereas cell rescue, detoxication and transcription factor-related genes were mostly up-regulated. We also observed an increased expression of polyamine and proline synthesis-related genes, as well as some carbohydrate-related genes such as sucrose synthase and trehalose-6-phosphate synthase.

ORGANISM(S): Solanum tuberosum

PROVIDER: GSE34110 | GEO | 2011/12/03

SECONDARY ACCESSION(S): PRJNA156491

REPOSITORIES: GEO

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Gene expression study after 3 days of cold exposure in a Solanum tuberosum cultivar called Desirée

Project description:The molecular response to cold exposure was studied in the leaves of an S. tuberosum clone using cDNA microarray. Differentially expressed genes were classified according to their known or predicted function and their expression ratio as compared to the control. The major changes upon cold exposure in Desirée leaves were observed in the photosynthesis-related genes, which were down-regulated, whereas cell rescue, detoxication and transcription factor-related genes were mostly up-regulated. We also observed an increased expression of polyamine and proline synthesis-related genes, as well as some carbohydrate-related genes such as sucrose synthase and trehalose-6-phosphate synthase. Gene expression was followed at day 3 after the beginning of the treatment: cold (4°C) and control conditions. For each condition, 4 independent biological replicates were sampled.

2011-12-03 | E-GEOD-34110 | biostudies-arrayexpress

Gene expression study after 3 days of salt exposure in a Solanum tuberosum cultivar called Desirée

Project description:The molecular response to salt exposure was studied in the leaves of an S. tuberosum clone using cDNA microarray. Differentially expressed genes were classified according to their known or predicted function and their expression ratio as compared to the control. The major changes upon a 150 mM NaCl exposure in potato leaves occurred in the photosystem apparatus and Calvin cycle: many transcripts coding for proteins belonging to photosystems I and II and chlorophyll synthesis were repressed, whereas cell rescue and transcription factor-related genes were mostly up-regulated. A strong down-regulation of several functional categories including primary metabolism, detoxication apparatus and signal transduction was also observed.

2011-12-03 | GSE34116 | GEO

Gene expression study after 3 days of salt exposure in a Solanum tuberosum cultivar called Desirée

2011-12-03 | E-GEOD-34116 | biostudies-arrayexpress

Gene expression study after 3 days of cold or salt exposure in a Solanum tuberosum cultivar called Desirée

Project description:This SuperSeries is composed of the following subset Series: GSE34110: Gene expression study after 3 days of cold exposure in a Solanum tuberosum cultivar called Desirée GSE34116: Gene expression study after 3 days of salt exposure in a Solanum tuberosum cultivar called Desirée Refer to individual Series

2011-12-03 | E-GEOD-34118 | biostudies-arrayexpress

Gene expression study after 1 and 3 days of salt exposure in a Solanum tuberosum cultivar called PS3

Project description:The molecular response to salt exposure was studied in the leaves of a S. tuberosum clone using cDNA microarray. Differentially expressed genes were classified according to their known or predicted function and their expression ratio as compared to the control. The major changes upon a 150 mM NaCl exposure in potato leaves occurred in the photosystem apparatus and Calvin cycle: many transcripts coding for proteins belonging to photosystems I and II and chlorophyll synthesis were repressed. On the other hand, we observed the induction of various kinds of transcription factors implicated in osmotic stress response via ABA-dependent or ABA-independent pathways but also in plant defense pathways. This revealed a crosstalk between abiotic and biotic stress responses during salt exposure, which activated several adaptation mechanisms including HSP, LEA, dehydrins and PR proteins. Gene expression changes related to carbohydrate and amino acid metabolism were also observed, pointing at putative modifications at the metabolic level.

2009-09-11 | GSE18053 | GEO

Gene expression study after 1 and 3 days of salt exposure in a Solanum tuberosum cultivar called PS3

2010-05-05 | E-GEOD-18053 | biostudies-arrayexpress

Solanum tuberosum cultivar:Delikat

Project description:Solanum tuberosum cv. 'Delikat leaves transcriptome

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Solanum tuberosum cultivar:Pirol

Project description:Solanum tuberosum cv. 'Pirol' leaves transcriptome

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Solanum tuberosum

Project description:Solanum tuberosum cold stress Transcriptome

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Transcriptomes profile of Solanum tuberosum (St) and Solanum commersonii (Sc) under cold stress and AtCBF3 overexpression.

Project description:St (common potato) is a freezing sensitive species unable to cold acclimate. The close wild relative Sc is freezing tolerant and able to cold acclimate. Here we compare the cold transcriptome of these two species with different levels of freezing tolerance. We also identify the putative CBF regulons by comparing the transcriptomes of wild type plants with that of 35S::AtCBF3 transgenic lines in both species. Plants were grown in 16:8 photoperiod. Eight hours after dawn, plants were either transfered to cold or kept in the warn. Wild type S. tuberosum and S. commersonii were grown at 2oC for 2h, 24h and 7 days. Wild type plants grown under warm temperatures for 2h was used as control for 2h cold samples; wild type warm grown plants for 24h were used as controls for 24h and 7 days cold samples. Under warm conditions, S. commersonii 35S::AtCBF3 lines were compared to S. commersonii wild type plants (same thing was done for S. tuberosum).

2011-04-30 | E-GEOD-22449 | biostudies-arrayexpress

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