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Photooxidative stress and light acclimation in the PSII antenna-deficient ch1 mutant

ABSTRACT: cea10-02_light - photooxidative stress - To characterize the metabolic pathways implicated in oxidative stress responses and in acclimation mechanisms in the ch1 mutant - The extracts are carried out starting from sheets of Arabidopis thaliana having pushed on compost in controlled conditions (light: 250 µmol.m-2.s-1, temperature: 22°C day 18°C night, moisture: 55%, photoperiod: 8:00 jour/16h night) during 4 and 8 weeks for respectively the Col0 genotype and the mutant ch1. The acclimatization of the plants is done during 48 H under an average luminous intensity (450 µmol.m-2.s-1, photoperiod 8:00), whereas the stress requires a strong luminous intensity (900 µmol.m-2.s-1, photoperiod 8:00) and low temperature (10°C day, 14°C night) also during 48 hours.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE34247 | GEO | 2011/12/09

SECONDARY ACCESSION(S): PRJNA149767

REPOSITORIES: GEO

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Project description:We combined heritability analysis of larval development rate with a global expression analysis of this phenotype to investigate genotype by environment interactions across three ecologically relevant temperatures in the Glanville fritillary butterfly (Melitaea cinxia). We focused upon the development of final instar caterpillars which is greatly affected by temperature, and during this stage the caterpillars build up most of the resources for adult life. Second generation, lab reared larvae, initially collected from the Åland metapopulation, were reared in standard lab condition until 6th larval instar. At the beginning of the final (7th) instar stage the larvae were separated into of three temperature conditions: Cold treatment (temperature profile: 8°C 18:00-9:59, 14°C 10:00-11:59, 20°C 12:00-15:59 and 14°C 16:00-17:59) Standard treatment (temperature profile: 15°C 17:00-6:59, 18°C 7:00-8:59, 22°C 9:00-10:59 and 26°C 11:00-16:59) Hot treatment (temperature profile: 8°C 20:00-7:59, 15°C 8:00-9:59, 35°C 10:00-17:59 and 15°C 18:00-19:59) The temperature profiles mimic the diurnal thermal variation of the natural habitat (Åland islands) of samples. Cold treatment mimics a cool and cloudy summer, Standard represents an average temperature profile in the Åland islands and Hot treatment mimics an exceptionally hot and sunny summer, with cold night-time temperatures. The experiment contained several larval families (full-sib) of which three were selected for gene expression analysis. Samples were snap-frozen in liquid nitrogen during mid-development (after 6, 5 and 4 days, for Cold, Standard and Hot respectively). Additional larvae from the same treatments were assayed for survival and growth. Gene expression was analyzed using a mixed model approach to identify genes with potential heritable expression variation (variation among families), genes with plastic expression responses (treatment induced changes) and genes with treatment dependent expression that varies among families (family by treatment interactions).

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Photooxidative stress and light acclimation in the PSII antenna-deficient ch1 mutant

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