Arabidopsis thaliana
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ABSTRACT:
PROVIDER: PRJNA149767 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:cea10-02_light - photooxidative stress - To characterize the metabolic pathways implicated in oxidative stress responses and in acclimation mechanisms in the ch1 mutant - The extracts are carried out starting from sheets of Arabidopis thaliana having pushed on compost in controlled conditions (light: 250 µmol.m-2.s-1, temperature: 22°C day 18°C night, moisture: 55%, photoperiod: 8:00 jour/16h night) during 4 and 8 weeks for respectively the Col0 genotype and the mutant ch1. The acclimatization of the plants is done during 48 H under an average luminous intensity (450 µmol.m-2.s-1, photoperiod 8:00), whereas the stress requires a strong luminous intensity (900 µmol.m-2.s-1, photoperiod 8:00) and low temperature (10°C day, 14°C night) also during 48 hours.
2011-12-09 | GSE34247 | GEO
Project description:Typically, when fully developed leaves of Arabidopsis thaliana are exposed to an increase in light intensity, they are able to increase their photosynthetic capacity in a process known as dynamic acclimation. Fully developed leaves of Arabidopsis thaliana were exposed to a fourfold increase in light intensity for 7 days to induce high light acclimation. This treatment was subjected to wild-type and a non-acclimating mutant lacking the gpt2 gene. The proteomic responses of the leaves were investigated using label-free mass spectrometry. A large reorganisation of the proteome was shown, with increases in the abundance of proteins of photosynthesis and carbon metabolism. Subtle differences were seen between the WT and gpt2 mutant: in the mutant, an increased stress response was seen, and some differences in the responses of metabolism. Proteomic responses generally correlated with physiological responses.
2017-11-14 | MSV000081715 | MassIVE
Project description:Typically, when fully developed leaves of Arabidopsis thaliana are exposed to an increase in light intensity, they are able to increase their photosynthetic capacity in a process known as dynamic acclimation. Fully developed leaves of Arabidopsis thaliana were exposed to a fourfold increase in light intensity for 7 days to induce high light acclimation. This treatment was subjected to wild-type and a non-acclimating mutant lacking the gpt2 gene. The proteomic responses of the leaves were investigated using label-free mass spectrometry. A large reorganisation of the proteome was shown, with increases in the abundance of proteins of photosynthesis and carbon metabolism. Subtle differences were seen between the WT and gpt2 mutant: in the mutant, an increased stress response was seen, and some differences in the responses of metabolism. Proteomic responses generally correlated with physiological responses.
2017-07-20 | PXD006598 | Pride
Project description:Plants are subjected to perpetual fluctuations of light intensity and spectral composition in their natural growth environment, particularly due to movement of clouds and upper canopy leaves. Sudden exposure to intense light is accompanied by absorption of excess light energy, which results in an overload of photosynthetic electron transport chain and generation of reactive oxygen species in and around thylakoid membranes. To cope with this photooxidative stress and to prevent chronic photoinhibition under dynamically changing light intensities, plants have evolved various short- and long-term photoprotective mechanisms. We used quantitative mass spectrometry to investigate long-term acclimation of Arabidopsis thaliana leaf proteome to fluctuating light (FL) which induces photooxidative stress. After three days of FL exposure the proteomes of young and mature leaves were analyzed separately in the morning and at the end of day to examine possible interaction between FL acclimation and leaf development or time of day.
2020-01-30 | PXD015330 | Pride
Project description:cea10-02_light - photooxidative stress - To characterize the metabolic pathways implicated in oxidative stress responses and in acclimation mechanisms in the ch1 mutant - The extracts are carried out starting from sheets of Arabidopis thaliana having pushed on compost in controlled conditions (light: 250 µmol.m-2.s-1, temperature: 22°C day 18°C night, moisture: 55%, photoperiod: 8:00 jour/16h night) during 4 and 8 weeks for respectively the Col0 genotype and the mutant ch1. The acclimatization of the plants is done during 48 H under an average luminous intensity (450 µmol.m-2.s-1, photoperiod 8:00), whereas the stress requires a strong luminous intensity (900 µmol.m-2.s-1, photoperiod 8:00) and low temperature (10°C day, 14°C night) also during 48 hours. 24 dye-swap - genotype comparison, treated vs untreated comparison
2011-12-09 | E-GEOD-34247 | biostudies-arrayexpress
Project description:Dynamic acclimation of photosynthesis plays an important role in increasing plant fitness under variable light environments. Acclimation is mediated by a glucose-6-phosphate/phosphate translocator, GPT2. This study examined whether plants lacking GPT2, which are defective in acclimation to increases in light, are more susceptible to oxidative stress. To understand this, we used the model plant of Arabidopsis thaliana (accession Wassilewskija-4 (Ws-4)) and compared this to mutants lacking GPT2. Plants were grown at low light (100 μmol m−2 s−1) for 7 weeks. For acclimation experiments, a set of plant from low light was transferred to 400 μmol m−2 s−1 for 7 days. Microarray analysis showed that gpt2 plants showed a greater induction of stress related genes relative to WT.
2021-03-24 | E-MTAB-10282 | biostudies-arrayexpress
Project description:Plants acclimate to environmental fluctuations by transitory reconfigurations the homeostatic network. Primary studies suggested that transcriptome responses to deal with fluctuations in light intensity and temperature tend to reversibility after stress removal in the model plant Arabidopsis thaliana. To gain more insight into this pattern in the context of acclimation, RNA-Seq analysis were conducted in Arabidopsis thaliana after different abiotic stress treatments consisting in high light (HL), high humidity, drought, heat, cold and combinations among factors or after recovery periods. Our transcriptome study is in line of a general pattern wherby transcriptome changes in response to adverse environments are prone to return to the basal state during the de-acclimation phase.
2023-06-28 | GSE226105 | GEO
Project description:Transcription profiling of Arabidopsis acclimation to light intensity
| PRJNA100669 | ENA
Project description:Exposure of mature fully expanded leaves of Arabidopsis to a 7.5 fold increased light intensity above growth light conditions (high light; HL) tirggers stress defensive responses but also initiates cellular processes, that if such conditions persist, can lead to increased photosynthetic capacity. This process is called dynamic acclimation. By using variational Bayesian state space modelling on eariler GEO deposited time series HL data (see GSE78251) a gene regulatory network of (co) transcription factor genes was inferred. The most connected gene in this network is BBX32, which was subequently shown to be a negative regulator of dynamic acclimation. Also present in the inferred network is HY5, which is known from studies on seedling photomorphogenesis to be antagonistic in its action to BBX32. Subsequently, it was demonstrated that HY5 is indeed a positive regulator of dynamic acclimation. This RNAseq-based study seeks to provide gene expression data that will help to link the immediate impact of these genes on the HL transcriptome to the longer term (several days) physiological manifestation of dynamic acclimation.
2021-04-01 | GSE158898 | GEO