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Retinoic acid (RA) induction time-course to profile gene expression during mES cell differentiation

ABSTRACT: Murine ES cell gene expression before RA induction are used to compare gene expression for time-points of 8, 12, 16, 24, 36, 48, 60 and 72 hours post-induction.

ORGANISM(S): Mus musculus

PROVIDER: GSE34279 | GEO | 2012/12/20

SECONDARY ACCESSION(S): PRJNA156525

REPOSITORIES: GEO

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Retinoic acid (RA) induction time-course to profile gene expression during mES cell differentiation

Project description:Murine ES cell gene expression before RA induction are used to compare gene expression for time-points of 8, 12, 16, 24, 36, 48, 60 and 72 hours post-induction. KH2 ES Cell RA Differentiation Time-course

2012-12-20 | E-GEOD-34279 | biostudies-arrayexpress

Retinoic acid (RA) induction time-course to profile gene expression during mES cell differentiation.

Project description:Murine ES cell gene expression before RA induction are used to compare gene expression for time-points of 2, 4, 6hrs post-induction.

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Retinoic acid (RA) induction time-course to profile gene expression during mES cell differentiation.

Project description:Murine ES cell gene expression before RA induction are used to compare gene expression for time-points of 2, 4, 6hrs post-induction. KH2 ES Cell RA Differentiation Time-course

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LOXL2 dependent genes in RA induced mES differentiation

Project description:Our results indicate that oxidation of TAF10 by LOXL2 induces its release from its promoters, leading to a block in TFIID-dependent gene transcription. Since TFIID complex is crucial for the expression of Nanog, Klf4, Sox2 and Oct4 and for maintaining the pluripotent state of embryonic stem cells, TAF10 oxidation by LOXL2 leads to inactivation of the pluripotency genes and a loss of pluripotent capacity in embryonic stem cells. Moreover, in vivo results demonstrate an essential role of LOXL2 in neural differentiation during zebrafish development: in the absence of LOXL2 the neural progenitor gene Sox2 is aberrantly overexpressed and neural differentiation is impaired. 12 samples were analyzed: mES transduced with control shRNA (n=3); mES transduced with LOXL2 shRNA (n=3), ES transduced with control shRNA and treated with RA (n=3); ES transduced with LOXL2 shRNA and treated with RA (n=3)

2015-12-21 | E-GEOD-65675 | biostudies-arrayexpress

Neural induction (Embrioid bodies-Retinoic acid)

Project description:Neural induction of ES by Retinoic acid Neural induction of ES by Retinoic acid

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Neural induction (Embrioid bodies-Retinoic acid)

Project description:Neural induction of ES by Retinoic acid Keywords: development or differentiation design,time series design

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Transcription profiling of mouse wild type vs CYP26A1-/- ES cells treated with control or 100 nM RA for 8 or 72 hr.

Project description:The goal of this study was to identify genes that are differentially expressed after genetic deletion of both alleles of the Cyp26a1 gene in murine embryonic stem cells. Cyp26a1 codes for the CYP26A1 enzyme which metabolizes RA to polar RA metabolites, such as 4-oxo-RA and 4-OH-RA. CYP26A1-/- ES cells do not metabolize RA within 48 hours of RA treatment while in Wt ES cells, polar RA metabolites are already detectable by 8 hr. In addition, the absence of CYP26A1 enzyme increases intracellular RA levels. By gene microarray analysis, we wanted to identify genes that would be affected by the lack of the Cyp26a1 gene. Experiment Overall Design: Wt and CYP26A1-/- ES cells were treated with control+LIF for 8 hours, and this was repeated 3 times. Wt and CYP26A1-/- ES cells were treated with 100 nM RA +LIF for 8 hours, and this was repeated 3 times. Wt and CYP26A1-/- ES cells were treated with 100 nM RA +LIF for 72 hours, and this was repeated 2 times.

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Retinoic Acid Is Sufficient for the in vitro Induction of Mouse Spermatocytes

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Retinoic Acid Is Sufficient for the in vitro Induction of Mouse Spermatocytes

Project description:To identify RA-regulated genes in mSSCs, we treated mSSCs with vehicle, RA and RA plus CHX. To characterize the induced spermatogenic cells by transcriptome profiling, we cultured mSSCs on pup Sertolic cells supplemented with RA. Induced differentiating spermatogonia and induced spermatocytes were collected after a 3-day- and 6-day-induction, respectively. We carried out RNA-seq analysis of samples after different treatment and samples from different in vitro induction stages.Two biological replicates were included for each sample.

2016-06-16 | E-GEOD-77051 | biostudies-arrayexpress

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Project description:The study consisted of two experiments. The hypothesis tested was that RA and tumor necrosis factor (TNF)-alpha would independently and synergistically regulate the expression of genes in THP-1 human myeloid cells, and that RA alone would be a significant modulator, as tested in a kinetic experiment.

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