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ATM kinase inhibition in glial cells activates the innate immune response and causes neurodegeneration in Drosophila

ABSTRACT: Analysis of the effects of ATM loss on gene expression to identify causes of neurodegeneration. ATM levels were reduced ubiquitously via the temperature-sensitive ATM^8 allele, or via tissue-specific RNAi of ATM (ATMi) using the Gal4/UAS expression system in neurons (Elav-GAL4 and ElavC155-GAL4) or glial cells (Repo-GAL4).

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE34315 | GEO | 2011/12/10

SECONDARY ACCESSION(S): PRJNA151497

REPOSITORIES: GEO

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ATM kinase inhibition in glial cells activates the innate immune response and causes neurodegeneration in Drosophila

Project description:Analysis of the effects of ATM loss on gene expression to identify causes of neurodegeneration. ATM levels were reduced ubiquitously via the temperature-sensitive ATM^8 allele, or via tissue-specific RNAi of ATM (ATMi) using the Gal4/UAS expression system in neurons (Elav-GAL4 and ElavC155-GAL4) or glial cells (Repo-GAL4). A 20-chip study using total RNA representing 2 replicates of 3 control genotypes (ATM^8/+, Repo-GAL4, and ElavC155-GAL4) and 3 experimental genotypes (ATM^8/ATM^8, Repo-ATMi, ElavC155-ATMi), and 4 replicates each of a control (Elav-GAL4) and experimental (Elav-ATMi) genotype

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The E3 Ligase CUL3 physically interacts with TANGO10 protein in vivo

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