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Nanog-like Regulates Endoderm Formation through the Mxtx2-Nodal Pathway

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Danio rerio

PROVIDER: GSE34684 | GEO | 2011/12/28

SECONDARY ACCESSION(S): PRJNA150309

REPOSITORIES: GEO

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Nanog-like regulates endoderm formation through the Mxtx2-Nodal pathway.

Project description:In mammalian embryonic stem cells, the acquisition of pluripotency is dependent on Nanog, but the in vivo analysis of Nanog has been hampered by its requirement for early mouse development. In an effort to examine the role of Nanog in vivo, we identified a zebrafish Nanog ortholog and found that its knockdown impaired endoderm formation. Genome-wide transcription analysis revealed that nanog-like morphants fail to develop the extraembryonic yolk syncytial layer (YSL), which produces Nodal, required for endoderm induction. We examined the genes that were regulated by Nanog-like and identified the homeobox gene mxtx2, which is both necessary and sufficient for YSL induction. Chromatin immunoprecipitation assays and genetic studies indicated that Nanog-like directly activates mxtx2, which, in turn, specifies the YSL lineage by directly activating YSL genes. Our study identifies a Nanog-like-Mxtx2-Nodal pathway and establishes a role for Nanog-like in regulating the formation of the extraembryonic tissue required for endoderm induction.

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Nanog-like Regulates Endoderm Formation through the Mxtx2-Nodal Pathway

Project description:This SuperSeries is composed of the following subset Series: GSE34682: Expression analysis of Nanog-like-MO1 injected zebrafish embryos at the sphere stage GSE34683: Genome-wide maps of binding sites of Nanog-like and Mxtx2 in blastula stage zebrafish embryos Refer to individual Series

2011-12-28 | E-GEOD-34684 | biostudies-arrayexpress

Nanog-like Regulates Endoderm Formation through the Mxtx2-Nodal Pathway

Project description:Nanog-like Regulates Endoderm Formation through the Mxtx2-Nodal Pathway

| PRJNA150309 | ENA