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Strome Pol II, H3K36me3 and CENP-A in met-1 EEMB

ABSTRACT: ChIP-chip of Pol II, H3K36me3 and CENP-A in met-1 C. elegans early embryo

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE35079 | GEO | 2012/01/24

SECONDARY ACCESSION(S): PRJNA150929

REPOSITORIES: GEO

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Strome Pol II, H3K36me3 and CENP-A in met-1 EEMB

Project description:ChIP-chip of Pol II, H3K36me3 and CENP-A in met-1 C. elegans early embryo EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: met-1(n4337); Developmental Stage: Early Embryo; Genotype: met-1(n4337); Sex: population predominantly Hermaphrodites perhaps with some Males; NUMBER OF REPLICATES: 2; EXPERIMENTAL FACTORS: temperature 20

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Project description:ChIP-chip of MES-4, H3K36me3 and H3K27me3 in mes-4 RNAi C. elegans early embryo EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: mes-4 RNAi; Developmental Stage: Early Embryo; Genotype: N2; Sex: population predominantly Hermaphrodites perhaps with some Males; NUMBER OF REPLICATES: 2; EXPERIMENTAL FACTORS: temperature 24

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CENP-A (Cnp1)

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CENP-T and CENP-C bridge adjacent CENP-A nucleosomes on young alpha-satellite dimers

Project description:We find that CENP-T acts as a bridge between two well-positioned CENP-A nucleosomes that are present on young alpha-satellite dimers that dominate functional human centromeres. CENP-T is centered over the CENP-B box, where it interacts with the CENP-B/CENP-C complex. Upon cross-linking, the entire CENP-A/CENP-C/CENP-T-containing complex is recovered as a nuclease-protected particle over an alpha-satellite dimer that comprises the fundamental unit of kinetochore chromatin. Our work reveals that CENP-A/CENP-C and CENP-T branches of kinetochore assembly are physically integrated.

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2012-11-06 | E-GEOD-35524 | biostudies-arrayexpress

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