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MiRNA profiling of double-positive thymocytes transduced with a retrovirus encoding Lin28a compared to untransduced controls

ABSTRACT: We used the NanoString mouse nCounter miRNA expression platform to compare the miRNA expression in double-positive (DP) thymocytes that developed in the presence (GFP+) or absence (GFP-ve) of ectopic mLin28 expression.

ORGANISM(S): Mus musculus

PROVIDER: GSE35081 | GEO | 2012/02/17

SECONDARY ACCESSION(S): PRJNA156163

REPOSITORIES: GEO

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miRNA profiling of double-positive thymocytes transduced with a retrovirus encoding Lin28a compared to untransduced controls

Project description:We used the NanoString mouse nCounter miRNA expression platform to compare the miRNA expression in double-positive (DP) thymocytes that developed in the presence (GFP+) or absence (GFP-ve) of ectopic mLin28 expression. Transduced (GFP+) and untransduced (GFP-ve) CD4+ CD8+ CD3- thymocytes were FACS sorted and pooled from three recipients of hematopoietic stem and progenitor cells transduced with Lin28-RV six weeks post-reconstitution (bone marrow chimeric mice). Total RNA was extracted and used for sample preparation and hybridization per manufacturer's recommendations.

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