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MiRNA profiling of double-positive thymocytes transduced with a retrovirus encoding Lin28a compared to untransduced controls

ABSTRACT: We used the NanoString mouse nCounter miRNA expression platform to compare the miRNA expression in double-positive (DP) thymocytes that developed in the presence (GFP+) or absence (GFP-ve) of ectopic mLin28 expression. Transduced (GFP+) and untransduced (GFP-ve) CD4+ CD8+ CD3- thymocytes were FACS sorted and pooled from three recipients of hematopoietic stem and progenitor cells transduced with Lin28-RV six weeks post-reconstitution (bone marrow chimeric mice). Total RNA was extracted and used for sample preparation and hybridization per manufacturer's recommendations.

ORGANISM(S): Mus musculus

SUBMITTER: Stefan Muljo

PROVIDER: E-GEOD-35081 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Lin28b reprograms adult bone marrow hematopoietic progenitors to mediate fetal-like lymphopoiesis.

Yuan Joan J Nguyen Cuong K CK Liu Xiuhuai X Kanellopoulou Chrysi C Muljo Stefan A SA

Science (New York, N.Y.) 20120216 6073

The immune system develops in waves during ontogeny; it is initially populated by cells generated from fetal hematopoietic stem cells (HSCs) and later by cells derived from adult HSCs. Remarkably, the genetic programs that control these two distinct stem cell fates remain poorly understood. We report that Lin28b is specifically expressed in mouse and human fetal liver and thymus, but not in adult bone marrow or thymus. We demonstrate that ectopic expression of Lin28 reprograms hematopoietic stem ...[more]

PMID: 22345399

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Lin28b expression distinguishes fetal versus adult lymphopoiesis

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