Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Development of gene expression profile in different tissues of zebra mussel and larvae

ABSTRACT: To analyze the different pattern of expression among tissues in D. polymorpha it was used acustom microarray designed in our laboratory using 4057 publicly availabe DNA sequences from Dreissena and other realted genera. Transcriptome profiles were analyszed using gills, digestive gland, gonad and half a body of adult zebra mussel and larvae 24 h pf. The samples analyzed in the microarray were not treated in order to detect the normal pattern of expression in each tissue. A total of 221 transcripts changed significantly their mRNA levels in the different tissues (ANOVA p<0.01, fc ±1.5). The results showed a clear separation on transcriptome profiles between samples with the main changes in gills, larvae and half a body.

ORGANISM(S): Dreissena polymorpha

PROVIDER: GSE35352 | GEO | 2012/01/27

SECONDARY ACCESSION(S): PRJNA152731

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Development of gene expression profile in different tissues of zebra mussel and larvae

Project description:To analyze the different pattern of expression among tissues in D. polymorpha it was used acustom microarray designed in our laboratory using 4057 publicly availabe DNA sequences from Dreissena and other realted genera. Transcriptome profiles were analyszed using gills, digestive gland, gonad and half a body of adult zebra mussel and larvae 24 h pf. The samples analyzed in the microarray were not treated in order to detect the normal pattern of expression in each tissue. A total of 221 transcripts changed significantly their mRNA levels in the different tissues (ANOVA p<0.01, fc ±1.5). The results showed a clear separation on transcriptome profiles between samples with the main changes in gills, larvae and half a body. Non treated animals were use to study different patterns of gene expression in different tissues using gills, digestive gland, gonad from both sexes, and half a body as well as larvae. It was used 2 replicate for each tissue.

2012-01-27 | E-GEOD-35352 | biostudies-arrayexpress

Factorial Microarray Analysis of Zebra Mussel Adhesion Process under the Impact of Multiple Environmental Factors

2009-07-10 | GSE16397 | GEO

Proteogenomic analysis of gills from the freshwater zebra mussel Dreissena polymorpha

Project description:RNA-seq informed proteomics of gills from Dreissena polymorpha

2023-09-15 | PXD031681 | Pride

Proteogenomics insights of hemocytes and plasma from the freshwater zebra mussel Dreissena polymorpha

Project description:RNA-seq informed proteomics of Dreissena polymorpha for discovering immune proteins from the zebra mussel hemolymph.

2019-04-25 | PXD012991 | Pride

Development of gene expression profile for a seasonal variation study in a natural population of Riba-Roja, in the Ebro River, Spain

Project description:To analyze the impact of seasonal and environmental variations on the physiology and metabolism of zebramussel, we use a custom microarray developed in our laboratory, using 4057 publicly availabe DNA sequences from Dreissena and other realted genera. Transcriptome profiles were analyszed using half-body samples captured in Riba Roja (low Ebro River), a location considered relatively free of chemical pollution, at three different stages of the vital cycle. The results showed a clear separation on transcriptome profiles between samples collected in February and June (pre- and spawning, respectively) and samples collected during gonad resorption (September). A total of 753 transcripts changed significantly their mRNA levels during these three months (ANOVA p<0.01, fc ±1.5). These changes could be considered as response to variations of temperature, reproductive stages, as well as food availability. Genes involved in oxidative stress were overrepresented in September, whereas genes related to reproductive functions were overrrepresented in the spawning and pre-spawning period. For each season (February: pre-spawning; June: Spawning; September: gonad resorption) it was used 2 biological replicates using the half body as a target tissue to analyze .

2012-01-27 | E-GEOD-35353 | biostudies-arrayexpress

Factorial Microarray Analysis of Zebra Mussel Adhesion Process under the Impact of Multiple Environmental Factors

Project description:Factorial Microarray Analysis of Zebra Mussel (Dreissena polymorpha) Adhesion Process under the Impact of Multiple Environmental Factors The expression profiles of the zebra mussel byssus unique genes in our cDNA microarray can be influenced by multiple factors. Three environmental factors plus adhesion status were considered as four main factors in this study. Two different levels in each factor were created, therefore, a 2x2x2x2 factorial experimental design was made by sixteen treatment groups with four biological replicates in each group. There were 32 dual-channel microarray slides included in this study and the effects of main factors on gene expression profiles as well as the two-way, three-way, and four-way interactions of the main factors were analyzed. Factors: temperature, water agitation, oxygen level, and adhesion status

2010-05-16 | E-GEOD-16397 | biostudies-arrayexpress

Development of gene expression profile for a seasonal variation study in a natural population of Riba-Roja, in the Ebro River, Spain

2012-01-27 | GSE35353 | GEO

Gene differential expression between attachment and detachment of zebra mussel

Project description:A zebra mussel byssus cDNA microarray was used to identify the differentially expressed genes between attachment and detachment. Keywords: Gene differential expression

2008-04-19 | GSE10851 | GEO

Mytilus edulis blue mussel larval development Control vs. Fatty acid diet deficiency

Project description:Blue mussel larvae were fed, in a first group, a balanced diet of essential fatty acids (EFAs) provided by a cocktail diet (COC) from three algal species. Larvae were cultured in three separate tanks from hatching, 0 day post-fertilization (DPF) until 42 DPF. Treated larvae were fed a deficient diet (Tiso) that contains low levels of arachidonic acid (AA) and eicosapentaenoic acid (EPA), two EFAs necessary for larval development, performance, and survival. The goal is to identify coordinated patterns of gene expression and understand their predictive function in relation to growth and mortality during early developmental stages of the blue mussel Mytilus edulis. In order to understand the mechanisms by which growth and survival drive an organism to the full range of its adaptation, we de novo assembled of the mussel transcriptome during early development using next-generation sequencing (NGS) technology, then designed customized microarrays targeting every developmental stage, which encompass major transitions in tissue organization of the fast-evolved blue mussel

2014-08-31 | GSE55580 | GEO

Identification of immune-related proteins from the freshwater zebra mussel Dreissena polymorpha.

Project description:RNA-seq informed proteomics of Dreissena polymorpha for discovering immune proteins involved in defence against protozoa and virus.

2020-04-15 | PXD017747 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data