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Mytilus edulis blue mussel larval development Control vs. Fatty acid diet deficiency

ABSTRACT: Blue mussel larvae were fed, in a first group, a balanced diet of essential fatty acids (EFAs) provided by a cocktail diet (COC) from three algal species. Larvae were cultured in three separate tanks from hatching, 0 day post-fertilization (DPF) until 42 DPF. Treated larvae were fed a deficient diet (Tiso) that contains low levels of arachidonic acid (AA) and eicosapentaenoic acid (EPA), two EFAs necessary for larval development, performance, and survival. The goal is to identify coordinated patterns of gene expression and understand their predictive function in relation to growth and mortality during early developmental stages of the blue mussel Mytilus edulis. In order to understand the mechanisms by which growth and survival drive an organism to the full range of its adaptation, we de novo assembled of the mussel transcriptome during early development using next-generation sequencing (NGS) technology, then designed customized microarrays targeting every developmental stage, which encompass major transitions in tissue organization of the fast-evolved blue mussel

ORGANISM(S): Mytilus edulis

PROVIDER: GSE55580 | GEO | 2014/08/31

SECONDARY ACCESSION(S): PRJNA240110

REPOSITORIES: GEO

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Mytilus edulis blue mussel larval development Control vs. Fatty acid diet deficiency

Project description:Blue mussel larvae were fed, in a first group, a balanced diet of essential fatty acids (EFAs) provided by a cocktail diet (COC) from three algal species. Larvae were cultured in three separate tanks from hatching, 0 day post-fertilization (DPF) until 42 DPF. Treated larvae were fed a deficient diet (Tiso) that contains low levels of arachidonic acid (AA) and eicosapentaenoic acid (EPA), two EFAs necessary for larval development, performance, and survival. The goal is to identify coordinated patterns of gene expression and understand their predictive function in relation to growth and mortality during early developmental stages of the blue mussel Mytilus edulis. In order to understand the mechanisms by which growth and survival drive an organism to the full range of its adaptation, we de novo assembled of the mussel transcriptome during early development using next-generation sequencing (NGS) technology, then designed customized microarrays targeting every developmental stage, which encompass major transitions in tissue organization of the fast-evolved blue mussel Two experimental conditions, COC and Tiso diets. Biological replicates 3 culture replicate per stage of development for 5 stages of development. Eggs and trocophore larvae did not undertake treatments

2014-08-31 | E-GEOD-55580 | biostudies-arrayexpress

RNA-seq data from the blue mussel (Mytilus edulis) gill samples in response to salinity and temperature

Project description:This project is aiming to examine the molecular response of the blue mussel (Mytilus edulis) to increased air temperatures and reduced salinity under laboratory conditions. There are 5 treatment groups (n=5), with group A representing the control (salinity 23percent salinity and temperature 5 degree celsius), group B ( 23percent salinity 30 degree celsius), group C (23percent salinity 33 degree celsius), group D (15percent salinity 5 degree celsius), group E (15percent salinity 30 degree celsius), group F (15percent salinity33 degree celsius), group G (5percent salinity 5 degree celsius).

2021-12-04 | E-MTAB-11178 | biostudies-arrayexpress

Environmental heat stress in Greenland blue mussels (Mytilus edulis)

Project description:Background biology: Global warming has accelerated in recent decades, with the Arctic warming 2–3 times faster than the global average. As a result boreal species are expanding into the Arctic, at a pace reflecting environmental warming. Nevertheless, the poleward expansion of boreal marine species is restricted by their ability to tolerate low water temperatures, and in the case of intertidal species, sub-zero air temperatures during winter. In Greenland, however, the number of days with extreme sub-zero air temperatures has decreased by more than 50% since the 1950’s, suggesting that the low air temperature constraint is weakening. Although boreal intertidal species could potentially benefit from this warmer climate to establish populations in the Arctic, recent work has shown that local intertidal summer air temperatures in Greenland can exceed 36°C. This temperature is above the thermoregulatory capacity of many boreal intertidal species, including the highly abundant blue mussel Mytilus edulis. Therefore will further colonisation of M. edulis in Greenland be inhibited by the increasingly warm summer temperatures. Aim of experiment: Intertidal animals (Greenland blue mussel M. edulis) were sampled in situ on the first warm days of the year from the inner (warmer) and outer (cooler) regions of the Godthåbsfjorden around Nuuk (64°N) to examine the fjord temperature gradient effect. In addition, subtidal M. edulis were also collected and subjected to two acute temperature shocks of 22 and 32°C, which represented common and extreme summer air temperatures for intertidal habitats near Nuuk.

2020-11-25 | E-MTAB-9786 | biostudies-arrayexpress

Mytilus edulis

Project description:Mytilus edulis blue mussel larval development Control vs. Fatty acid diet deficiency

| PRJNA240110 | ENA

Alteration of Gene Expression Profile in Spontaneously Hypertensive Rats Treated with Protein Hydrolysate of Blue Mussel (Mytilus edulis) by DNA Microarray Analysis

Project description:The aim of this study was to investigate the antihypertensive effect of enzymatic hydrolysis of blue mussel protein (HBMP) in rats. Spontaneously hypertensive rats (SHRs) were orally administration with high- or low-dose of HBMP for 28 days. Major components of the renin-angiotensin (RAS) system in serum of SHRs from different groups were analyzed, and gene expression profiling were performed in the kidney of SHRs, using the Whole Rat Genome Oligonucleotide Microarray. Results indicated although genes involved in RAS system were not significantly altered, those related to blood coagulation system, cytokine and growth factor, and fatty acids metabolism were remarkablely changed. Several genes which were seldom reported to be implicated in pathogenesis of hypertension also showed significant expression alterations after oral administration of HBMP.

2016-09-01 | GSE73387 | GEO

Proteomic investigation of the blue mussel larval shell organic matrix

Project description:This study aims to characterize the larval shell proteome of the blue mussel for the first time and to compare it to adult mussel shell proteomes

2022-03-17 | PXD013675 | Pride

RNA-seq data from the blue mussel (Mytilus edulis) gill samples in response to salinity and temperature

Project description:RNA-seq data from the blue mussel (Mytilus edulis) gill samples in response to salinity and temperature

| PRJEB48959 | ENA

Molecular mechanisms underlying heat stress effects on Mytilus edulis and galloprovincialis hybrids at early larval stages

Project description:The present work sought first to identify the impacts of increasing water temperatures on M. galloprovincialis and M. edulis pure larvae and their hybrids on embryo larval development. Second, based on a recently developed targeted Mussel’s microarray, we investigated the transcriptional response to elucidate possible differences in heat stress-induced gene expression between these species.

2020-05-31 | GSE128849 | GEO

Hypoxia-reoxygenation stress response in the marine facultative anaerobe Mytilus edulis

Project description:Hypoxia is a characteristic feature of marine environments and a major stressor for marine organisms inhabiting benthic and intertidal zones. Several studies have explored the responses of these organisms to hypoxic stress at the whole organism level with a focus on energy metabolism and mitochondrial response, but the instrinsic mitochondrial responses that support the organelle’s function under hypoxia and reoxygenation (H/R) stress are not well understood. We studied the effects of acute H/R stress (10 min anoxia followed by 15 min reoxygenation) on mitochondrial respiration, production of reactive oxygen species (ROS) and posttranslational modifications (PTM) of the proteome in a marine facultative anaerobe, the blue mussel Mytilus edulis. The mussels’ mitochondria showed increased OXPHOS respiration and suppressed proton leak resulting in a higher coupling efficiency after H/R stress. ROS production decreased in both the resting (LEAK) and phosphorylating (OXPHOS) state indicating that M. edulis is able to prevent oxidative stress and mitochondrial damage during reoxygenation. Hypoxia did not stimulate the rearrangement of the mitochondrial supercomplexes but impacted the mitochondrial phosphoproteome including the proteins involved in OXPHOS, amino acid and fatty acid catabolism, and protein quality control. This study indicates that mussels’ mitochondria possess intrinsic mechanisms (including regulation via PTM mechanisms such as reversible protein phosphorylation) that ensure high respiratory flux and mitigate oxidative damage during H/R stress and contribute to the hypoxia-tolerant mitochondrial phenotype of this metabolically plastic species.

2021-11-25 | PXD027955 | Pride

Transcriptomic and Network Analyses Reveals Mechanistic-Based Biomarkers of Endocrine Disruption in the Marine Mussel, Mytilus edulis

Project description:Coastal monitoring in the 21st century faces challenges as measuring exposure using traditional toxicity approaches is not practical or financially feasible for the numerous contaminants entering oceans. Transcriptomics, high-throughput assays, and adverse outcome pathways (AOP) have been one approach to overcome these obstacles but development of these methods is challenging for non-model organisms and contaminants with unknown mechanisms. Endocrine Disrupting Compounds (EDCs) have been found to cause reproductive impairments such as intersex in bivalves, however, the mechanism linked to this adverse outcome (AO) is unknown as the estrogen receptor (ER) has been identified to be unresponsive to EDCs. To develop mechanism-based biomarkers that may be linked through an AOP we exposed Mytilus edulis to 17-alpha-ethinylestradiol (5 and 50ng/L) and 4-nonylphenol (1 and 100μg/L), and performed gene expression analysis on digestive gland tissue using a M. edulis microarray. Through network and targeted analyses, we identified the non-genomic estrogen signaling pathway and steroidogenesis pathway as the likely mechanism of action and identified biomarkers and potential key events (KE) for an AOP of endocrine disruption in marine mussels. The mechanism-based biomarkers were validated in the laboratory, tested in the field, and were responsive in areas known to be contaminated with EDCs.

2018-03-15 | GSE111836 | GEO

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