Transcriptomics

Dataset Information

0

Comparison of gene expression profiles of p45-/- primary megakaryocytes and WT primary megakaryocytes


ABSTRACT: Whole livers were collected from mouse fetuses at embryonic day 13.5 (E13.5), and single-cell suspensions were prepared by successive passage through 25-gauge needles. Fetal liver cells were maintained in RPMI1640 (Wako, Osaka, Japan) supplemented with 20% charcoal-stripped fetal bovine serum (FBS), 100 U/ml penicillin, 100µg/ml streptomycin, and 50ng/ml recombinant human thrombopoietin (TPO) (generously provided by Kyowa Hakko Kirin Co. Ltd.). Megakaryocytes were harvested for RNA purification from a day-3 culture. CD41+ cells were enriched using biotinylated anti-CD41 antibody (Serotec; clone MWReg30) and streptavidin-coupled Dynabeads (Dynal Biotech ASA) from a culture of E13.5 fetal liver cells of WT and p45–/– mice. Total RNA was purified from the sorted CD41+ cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE35645 | GEO | 2013/08/07

SECONDARY ACCESSION(S): PRJNA152501

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2013-08-07 | E-GEOD-35645 | biostudies-arrayexpress
2010-03-22 | E-GEOD-15581 | biostudies-arrayexpress
2014-06-11 | GSE52141 | GEO
2020-07-15 | GSE125579 | GEO
2004-09-17 | GSE997 | GEO
2014-07-27 | E-MTAB-2625 | biostudies-arrayexpress
2021-01-04 | GSE152574 | GEO
2008-06-17 | E-GEOD-997 | biostudies-arrayexpress
2003-12-25 | GSE567 | GEO
2023-01-01 | GSE165326 | GEO