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Enigma (CG9006) RNAi vs control RNAi in Drosophila Kc-167 cells.

ABSTRACT: 5 day RNAi treatment to knockdown Enigma, CG9006, a Drosophila mitochondrial protein with homology to acyl-CoA dehydrogenases. Keywords: Genetic modification

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE3566 | GEO | 2006/02/04

SECONDARY ACCESSION(S): PRJNA93639

REPOSITORIES: GEO

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RNAi knock down of Enigma, a mitochondrial protein with homology to acyl-CoA dehydrogenases, in Drosophila

Project description:5 day RNAi treatment to knockdown Enigma, CG9006, a Drosophila mitochondrial protein with homology to acyl-CoA dehydrogenases. Experiment Overall Design: Kc-167 cells, 5 day treatment with Enigma-specific RNAi or beta-lactamase control RNAi. Specifics:1.6X10^6 cells in 0.5mL of serum-free Sang's M3 media incubated for 1 hour with 20ug of dsRNA, subsequently equal volume of M3 media containing 10% serum was added. Cells harvested after 5 days. RNAi efficiency measured by immunoblot for Enigma.

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Project description:Transcription profiling by array of Drosophila Kc167 cells with RNAi knockdown of Enigma, a mitochondrial protein with homology to acyl-CoA dehydrogenases

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