Transcriptomics

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Expression analysis with Phospholipase C over expression


ABSTRACT: The regulation of phospholipid biosynthesis in Saccharomyces cerevisiae through cis-acting upstream activating sequence inositol (UASino) and trans-acting elements, such as the INO2-INO4 complex and OPI1 by inositol supplementation in growth is thoroughly studied. In this study, we provide evidence for the regulation of lipid biosynthesis by phosphatidylinositol-specific phospholipase C (PLC) through UASino and two trans-acting elements. Gene expression analysis and radiolabelling experiments demonstrated that the overexpression of rice PLC in yeast cells altered phospholipid biosynthesis at the levels of transcriptional and enzyme activity.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE35745 | GEO | 2012/09/01

SECONDARY ACCESSION(S): PRJNA152207

REPOSITORIES: GEO

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