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Gene expression changes induced in the stromal cell line EL08-1D2 by co-culture with leukemic B cells (CLL)

ABSTRACT: Tumor cell survival critically depends on heterotypic communication with benign cells in the microenvironment. Here we describe a novel survival signaling pathway activated in stromal cells by contact to B-cells from chronic lymphocytic leukemia (CLL) patients. The expression of PKC-βII and the subsequent activation of NF-κB in bone marrow stromal cells is a prerequisite to support the survival of malignant B-cells. PKC-β knockout mice are insusceptible to CLL-transplantations, underscoring the in vivo significance of the PKC-βII- NF-κB signaling pathway in the tumor microenvironment. Upregulated stromal PKC-βII in biopsies from CLL, breast- and pancreatic- cancer patients suggest that this pathway may commonly be activated in a variety of malignancies. We used microarrays to determine gene expression changes induced by co-culturing with leukemic B-cells (CLL) in EL08-1D2 cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE36414 | GEO | 2013/03/08

SECONDARY ACCESSION(S): PRJNA155537

REPOSITORIES: GEO

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Effect of NF-kappaB activation in bone marrow stromal cells co-cultured with CLL cells

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