Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expresion profile of MEF reprogrammed with Yamanaka´s factor together with FoxA2 and Gata4

ABSTRACT: In a pilot experiment to reprogramme MEF into endoderm, we infected MEF with the Yamanaka´s factors (O: Oct4, K: Klf4, S: Sox2, M:Myc), FoxA2 (F) and Gata4 (G). Global gene expression of isolated clones was performed.

ORGANISM(S): Mus musculus

PROVIDER: GSE37548 | GEO | 2012/08/30

SECONDARY ACCESSION(S): PRJNA161541

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expresion profile of MEF reprogrammed with YamanakaM-BM-4s factor together with FoxA2 and Gata4

Project description:In a pilot experiment to reprogramme MEF into endoderm, we infected MEF with the YamanakaM-BM-4s factors (O: Oct4, K: Klf4, S: Sox2, M:Myc), FoxA2 (F) and Gata4 (G). Global gene expression of isolated clones was performed. After infection of MEF with the YamanakaM-BM-4s factor (OKS/M), FoxA2 and Gata4, the cells were grown until colonies could be isolated and expanded independently. Global gene expression of several clones was performed.

2012-08-30 | E-GEOD-37548 | biostudies-arrayexpress

Transcriptome analysis of cells of different cycling speed during Yamanaka reprogramming

Project description:Reprogramming somatic cells to induced pluripotency by Yamanaka factors is usually slow and inefficient, and is thought to be a stochastic process. We identified a privileged somatic cell state, from which acquisition of pluripotency could occur in a non-stochastic manner. Subsets of murine hematopoietic progenitors are privileged, whose progeny cells predominantly adopt the pluripotent fate with activation of endogenous Oct4 locus after 4-5 divisions in reprogramming conditions. Privileged cells display an ultrafast cell cycle of ~8 hours. In fibroblasts, a subpopulation cycling at a similar ultrafast speed is observed after 6 days of factor expression, and is increased by p53-knockdown. This ultrafast-cycling population accounts for >99% of the bulk reprogramming activity in wildtype or p53-knockdown fibroblasts. We compared the transcriptomes of the fast cycling cells with those of slower hematopoietic progenitors, bulk fibroblasts and established iPS cells. 3-5 replicates for each of the six cell types were included: 4 replicates for established iPS cells, 4 replicates for bulk mouse embryonic fibroblasts (MEF), 4 replicates for fast cycling MEF, 4 replicates for slow cycling MEF, 5 replicates for fast cycling granulocyte monocyte progenitors (GMP) and 3 replicates for slow cycling GMP.

2014-02-12 | E-GEOD-53074 | biostudies-arrayexpress

Comparison of the gene expression profiles between iPS-OSH, iPS-J, ESC and MEF.

Project description:iPS-OSH was the novel iPS cells generated from our lab, the microarray analysis was used for determining the gene expression profiles of iPS-OSH. iPS-J was provided from Dr. Yamanaka (Riken, APS0001). Microarray analysis was used to compare the difference of gene expression profiles in iPS-OSH, iPS-J, ESC and MEF. The total RNA extracted from iPS-OSH, iPS-J, ESC and MEF was used for the microarray analysis to compare the gene expression profiles.

2012-07-31 | E-GEOD-31822 | biostudies-arrayexpress

Comparison of the gene expression profiles between iPS-OSH, iPS-J, ESC and MEF.

2012-08-01 | GSE31822 | GEO

iTRAQ-based quantitative proteomic analysis of Yamanaka factors reprogrammed breast cancer cells.

Project description:Cancer cells had been developed to be reprogrammed into embryonic stem like cells by induced pluripotent stem cells (iPSCs) technology, however, the tumor differentiation/dedifferentiation mechanisms had not yet been analyzed on a genome-wide scale. Here, we inserted the four stem cell transcription factor genes OCT4, SOX2, C-MYC and KLF4 into MCF cells (MCFs), represented a female breast cancer cell type, and obtained iPSCs (Mcfips) in about 3 weeks. By using the LC MS/MS iTRAQ technology, we analyzed the proteomic changes between MCFs and Mcfips. Of identified 4,616 proteins totally, 247 and 142 differentially expressed (DE) proteins were found in Mcfips compared with human induce pluripotent stem cells (Hips) and MCFs, respectively. 35 co-up and 10 co-down regulated proteins were recognized in DE proteins. Above DE proteins were categorized with GO functional classification annotation and KEGG metabolic pathway analysis into biological processes. In the protein interaction network, we found 37 and 39 hubs interacted with more than one protein in Mcfips comparing to Hips, in addition, 25 and 9 hubs were identified in Mcfips comparing to MCFs. Importantly, the mitochondria, ribosome and tumor suppressor proteins were found to be core regulators of tumor reprogramming, which might contribute to understand the mechanisms in relation to the occurrences and progression of a tumor. Thus, our study provided a valuable data for exploring the possibility to normalize the malignant phenotype.

| S-EPMC5470971 | biostudies-literature

Nrdc-deficient MEF

Project description:Nrdc-deficient MEF

| PRJNA354417 | ENA

Reconstituted Rela-/- MEF

Project description:Reconstituted Rela-/- MEF

| PRJNA549298 | ENA

Comparison of primary MEF vs HRasV12 + Twist transformed MEF

Project description:MEF cells were sequentially infected with H-RasV12 and Twist (Twist1 or Twist2) expression retroviral constructs. Gene expression profiles of the resulting transformed cell lines were compared to the gene expression profile of primary MEF cells

2008-07-11 | GSE11756 | GEO

Irradiation response in PKBalpha MEF

Project description:MEF proficient or deficient in PKBalpha, or PKBalpha knockout MEF where PKBalpha expression was reconstituted by stable transfection were subjected to 10Gy gamma-IR treatment. 4 or 24 hours after treatment, gene expression changes were analyzed. Keywords: genotype0-specific stress response in PKBalpha MEF

2008-04-04 | GSE9146 | GEO