Exon-level expression profiling in MDA-MB-231 with or without TIP-1 expression
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE37555 | GEO | 2014/04/18
SECONDARY ACCESSION(S): PRJNA161523
REPOSITORIES: GEO
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Project description:MDA-MB-231 stable cell lines transfected with control shRNA or TIP-1 shRNA were cultured overnight. Total RNA of these cell lines was isolated for microarray analysis. Try to get more information about the difference in the mRNA levels in these cell lines. We analyzed gene expression from 6 samples: 2 control MDA-MB-231 (two independent clones) and 4 shRNA TIP-1 MDA-MB-231 cells (2 different shRNAs to silence TIP-1 expression, each shRNA has two independent clones). We used the Affymetrix Human Gene 1.0 ST platform. Array data was processed by the Affymetrix Exon Array Computational Tool. No technical replicates were performed.
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Project description:D54 stable cell lines that transfected with control shRNA and TIP-1 shRNA were cultured overnight. Isolated the total RNA of these cell lines to do microarray. Try to get more information about the difference in the mRNA level in these cell lines. We analyzed gene expression from 5 subjects: 2 control D54 (two independent clones) and 3 shRNA TIP-1 D54 cells (3 differenct shRNA to scilence TIP-1 expression) using the Affymetrix Human Exon 1.0 ST platform. Array data was processed by Affymetrix Exon Array Computational Tool. No techinical replicates were performed.
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