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Role of miR-146a in colon cancer cells

ABSTRACT: miRNAs exert various biological functions by targeting different cellular targets. Studying miR-146a functions in colon cancer cells helps to understand colorectal cancer (CRC) malignancy and progression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE37596 | GEO | 2013/11/20

SECONDARY ACCESSION(S): PRJNA162559

REPOSITORIES: GEO

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Role of miR-146a in colon cancer cells

Project description:miRNAs exert various biological functions by targeting different cellular targets. Studying miR-146a functions in colon cancer cells helps to understand colorectal cancer (CRC) malignancy and progression. HT29 miR-146a-expressing cells and empty control cells were established for mining the gene expression profiles upon miR-146a expression.

2013-11-20 | E-GEOD-37596 | biostudies-arrayexpress

miR-146a Exerts Differential Effects on Melanoma Growth and Metastatization.

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miR-146a target identification in gastric cancer

Project description:We have found expression of miR-146a up-regulated in gastric cancer. To identify new targets of miR-146a we profiled the transcriptome after miR-146a over-expression in the human gastric cancer cell line SNU638. SNU638 cells were transfected in triplicates with 50 nM miR-146a or control (siGlo) using Lipofectamine 2000. Total RNA was harvested 24 h after transfection using Trizol reagent. There are a total of six arrays included in this experiment, including three biological replicates of mRNA expression after miR-146a over-expression and three controls in SNU638 cells.

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MicroRNA-146a modulates B-cell oncogenesis by regulating EGR1

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Project description:MiR-146a is an important regulator of innate inflammatory responses and is also implicated in cell death and survival. Here, we identified microglia as the main cellular source of miR-146a among mouse CNS resident cells. We further characterized the phenotype of miR-146a KO microglia cells during in vivo demyelination induced by cuprizone (CPZ) and found reduced number of CD11c+ microglia in the KO compared to WT mice. Microglia were also isolated from the brain, and the proteome was analyzed by liquid chromatography mass spectrometry.

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Global transcriptome profiling was performed for keratinocytes transfected with either miR-146a mimics (Pre-146a) or negative control (Pre-NC).

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