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Expression data from one-year-old leaves of Populus simonii treated with short-term water deficit

ABSTRACT: We take one-year-old plants for short-term water deficit treatments and controls. We use the Affymetrix Poplar GeneChip to decrypt the gene functions and mechanisms in Populus simonii leaves and detail the global program of gene expression during water deficit treatments.

ORGANISM(S): Populus sp. Populus simonii

PROVIDER: GSE37608 | GEO | 2012/04/27

SECONDARY ACCESSION(S): PRJNA162395

REPOSITORIES: GEO

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Expression data from one-year-old leaves of Populus simonii treated with short-term water deficit

Project description:We take one-year-old plants for short-term water deficit treatments and controls. We use the Affymetrix Poplar GeneChip to decrypt the gene functions and mechanisms in Populus simonii leaves and detail the global program of gene expression during water deficit treatments. Populus simonii leaves were taken from short-term water-deficit-treated plants and control plants for RNA extraction and hybridization on Affymetrix microarrays. D1 and D2 are from water-deficit-treated plants, CK1 and CK2 are controls.

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Project description:We take the one year old plant for 100 mMol/L NaCl treatments 24 hours and controls. Use the Affymetrix poplar gene chip to decrypt the gene functions and mechanisms in Populus simonii leaves. We used microarrays to detail the global programme of gene expression during NaCl treatments. Populus simonii leaves were taken from 100 mMol/L NaCl treatments 24 hours and controls for RNA extraction and hybridization on Affymetrix microarrays.S1, S2 and S3 from NaCl treatments, CK1, CK2 and CK3 controls.

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Expression data from one year old leaves of Populus simonii by chilling stress (4°C, 10h).

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Expression data from one year old leaves of Populus simonii by chilling stress (4M-BM-0C, 10h).

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affy_popsec_nancy_leaves2008_poplar -Molecular bases of acclimatation and adaptation to water deficit in poplar

Project description:affy_popsec_nancy_leaves_poplar - affy_popsec_nancy_leaves2008_poplar - This project aims to identify genes of interest for water deficit acclimation and/or adaptation in a tree species: poplar. We look for genes and gene expression networks related to drought stress. We intend to analyse the transcriptome in mature leaves, in two genotypes, Carpaccio and Soligo, at various stages and intensities of stress. During water deficit, leaves underwent many processes aiming to maintain cells integrity such as water status adjustment through osmoregulation or cell detoxication. These analyses intend to identify genes of interest involved in homeostasis maintenance. The comparison between medium and severe stress intensities and between early and long term stresses will power the selection of genes of interest. The co-analysis of two genotypes of contrasted tolerance to water deficit should help to discriminate genes presenting a potential adaptative character from genes responding passively to the constraint-In a first experiment, two poplar clones, Soligo (S) and Carpacio (C) were submitted to 4 treatments: control, mild water deficit, moderate water deficit (12-day long for both) and early-drought stress (about 36-h long). Growth and physiology was characterised on a batch of plants and samples collected on another batch of plants. In a second experiment, two poplar clones, Soligo (S) and Carpacio (C) were submitted to 2 treatments: control and moderate water deficit. Mature leaves were collected and total RNAs were extracted from each tree individually. Two pools of 3 (or 2) individuals were made using equimolar ratio. A pool is considered as one biological replicate and corresponds to one Affymetrix slide. Keywords: treated vs untreated comparison

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