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Transcriptomics of coping strategies in free-swimming Lepeophtheirus salmonis (Copepoda)

ABSTRACT: To understand the sensitivity of larval lice to changing environmental parameters we applied a 38K oligo microarray and characterized transcriptome responses after 24 hour exposures to reduced salinity (30-10 parts per thousand (ppt)) or varied temperature (16-4ºC). To further characterize the effects of salinity, we profiled expression changes over a range of seawater with single increment differences (30-25 ppt).

ORGANISM(S): Lepeophtheirus salmonis

PROVIDER: GSE37976 | GEO | 2012/05/19

SECONDARY ACCESSION(S): PRJNA167141

REPOSITORIES: GEO

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Transcriptomics of coping strategies in free-swimming Lepeophtheirus salmonis (Copepoda)

Project description:To understand the sensitivity of larval lice to changing environmental parameters we applied a 38K oligo microarray and characterized transcriptome responses after 24 hour exposures to reduced salinity (30-10 parts per thousand (ppt)) or varied temperature (16-4M-BM-:C). To further characterize the effects of salinity, we profiled expression changes over a range of seawater with single increment differences (30-25 ppt). Three separate projects. Low resolution salinity (aka wide range or LR_SAL) contains 2 duplicate experiments, each with pools of lice (~500 copepodid lice per beaker; n=3 beakers per condition), incubated for 24 hr at 10M-BM-:C diluted to 30, 25, 20, or 10 parts per thousand (ppt). Temperature study conducted as above, but with a constant salinity of 30 ppt and changed temperature of 4, 10, or 16M-BM-:C. High resolution salinity conducted as above, but with no experiment replication, but with biological repication of n=6 beakers per condition, and with a range of 25, 26, 27, 28, 29, 30 ppt salinity. Please note that each experiment was normalized separately.

2012-05-18 | E-GEOD-37976 | biostudies-arrayexpress

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Transcriptomics of coping strategies in free-swimming Lepeophtheirus salmonis (Copepoda) larvae responding to abiotic stress.

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