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An overlapping protein-coding region in Influenza A Virus Segment 3 modulates the host response

ABSTRACT: Segment 3 of influenza A virus contains a second open reading frame accessed via robosomal frameshifting. The frameshift product, PA-Z, comprises the endonuclease domain of viral PA protein with C-terminal demain encoded by the X-ORF and functions to repress cellular gene expression. PA-X also modulates IAV virulence in a mouse infection model.

ORGANISM(S): Mus musculus

PROVIDER: GSE38112 | GEO | 2012/07/12

SECONDARY ACCESSION(S): PRJNA167288

REPOSITORIES: GEO

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An overlapping protein-coding region in Influenza A Virus Segment 3 modulates the host response

Project description:Segment 3 of influenza A virus contains a second open reading frame accessed via robosomal frameshifting. The frameshift product, PA-Z, comprises the endonuclease domain of viral PA protein with C-terminal demain encoded by the X-ORF and functions to repress cellular gene expression. PA-X also modulates IAV virulence in a mouse infection model. The effect of PA-X deltions were examined using 2 separate mutant influenza viruses (1918-FS/1918-PCT1) and the results compared to wild-type 1918 influenza virus. Balb/c mice were infected and samples harvested at days 3, 5 and 8 days. Four animals/condition were used. Gene expression levels from infected animals were compared to mock animals.

2012-07-11 | E-GEOD-38112 | biostudies-arrayexpress

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The dynamic proteome of influenza A virus infection identifies M segment splicing as a host range determinant

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Systematic analysis of non programmed frameshift suppression in E. coli via translational tiling proteomics

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