Transcriptomics

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GRO-seq from HCT116 cells


ABSTRACT: The majority of transcription studies examine steady-state RNA . However steady-state RNA is not a true reflection of the transcriptome, because the RNA levels are affected by both transcription rate and degradation rate. In this experiment we measured the amount of transcription occurring in HCT116 colon cancer cells, regardless of degradation, using GRO-seq (global nuclear run-on sequencing). This information demonstrates that many genes have a pile-up of transcriptionally-engaged polymerase near their 5'-end.

ORGANISM(S): Homo sapiens

PROVIDER: GSE38140 | GEO | 2013/03/01

SECONDARY ACCESSION(S): PRJNA167277

REPOSITORIES: GEO

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