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GRO analysis of rap1-sil, tpk1 & tpk2.

ABSTRACT: GRO (Genomic run-on) experiments with different mutants that affect to the accumulation of non active RNA pol II along the yeast genome. Keywords: Genomic run-on GRO There are 4 different strains: rap1-sil (without the silencing domain), RAP1(both from Graham I.R. et al 1999) and tpk1 & tpk2 mutants (Euroscarf). For each experiment there are GRO data (Transcription Rate) and gDNA data used for normalizing the GRO signals. The last number of the GRO filters correspond to the number of gDNA filters.There are 3 independent biological replicates of Rap1 and rap1-sil experiments and 2 for the tpk1 & tpk2 experiments.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jose Perez-Ortin

PROVIDER: E-GEOD-14080 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulon-specific control of transcription elongation across the yeast genome.

Pelechano Vicent V Jimeno-González Silvia S Rodríguez-Gil Alfonso A García-Martínez José J Pérez-Ortín José E JE Chávez Sebastián S

PLoS genetics 20090821 8

Transcription elongation by RNA polymerase II was often considered an invariant non-regulated process. However, genome-wide studies have shown that transcriptional pausing during elongation is a frequent phenomenon in tightly-regulated metazoan genes. Using a combination of ChIP-on-chip and genomic run-on approaches, we found that the proportion of transcriptionally active RNA polymerase II (active versus total) present throughout the yeast genome is characteristic of some functional gene classe ...[more]

PMID: 19696888

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