Transcriptomics

Dataset Information

0

RNA sequencing revealed novel actors of the acquisition of drug resistance in Candida albicans


ABSTRACT: Drug susceptible clinical isolates of Candida albicans frequently become highly tolerant to drugs during chemotherapy, with dreadful consequences on patient health. We used RNA sequencing (RNA-seq) to analyze the transcriptomes of a CDR (Candida Drug Resistance) strain and its isogenic drug sensitive counterpart. RNA-seq unveiled differential expression of 228 genes including a) genes previously identified as involved in CDR, b) genes not previously associated to the CDR phenotype, and c) novel transcripts whose function as a gene is uncharacterized. In particular, we show for the first time that CDR acquisition is correlated with an overexpression of the transcription factor encoding gene CZF1. CZF1 null mutants were sensitive to many drugs, independently of known multidrug resistance mechanisms. We show that CZF1 acts as a repressor of β-glucan synthesis, thus negatively regulating cell wall integrity. Finally, our RNA-seq data allowed us to identify a new transcribed region, upstream of the TAC1 gene, which encodes the major CDR transcriptional regulator. Our results open new perspectives to the role of Czf1 and to our understanding of the transcriptional and post-transcriptional mechanisms that lead to the acquisition of drug resistance in C. albicans, with potential future improvements of therapeutic strategies.

ORGANISM(S): Candida albicans

PROVIDER: GSE38298 | GEO | 2012/09/04

SECONDARY ACCESSION(S): PRJNA167631

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2012-09-04 | E-GEOD-38298 | biostudies-arrayexpress
2017-08-01 | GSE76858 | GEO
2016-10-27 | E-SYBR-13 | biostudies-arrayexpress
2014-07-21 | E-GEOD-57451 | biostudies-arrayexpress
| PRJNA324272 | ENA
2024-10-06 | GSE267941 | GEO
2024-10-06 | GSE267940 | GEO
2010-05-25 | E-GEOD-8727 | biostudies-arrayexpress
2014-07-21 | GSE57451 | GEO
2017-07-18 | GSE60121 | GEO