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Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae FLB4-deleted mutant and its parental strain 70-15

ABSTRACT: Genes potentially regulated by the Flb4p transcription factor were identified by microarray-based transcriptome analysis of the flb4- mutant and the parental strain 70-15.

ORGANISM(S): Pyricularia grisea Pyricularia oryzae

PROVIDER: GSE38328 | GEO | 2013/01/01

SECONDARY ACCESSION(S): PRJNA167718

REPOSITORIES: GEO

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Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae FLB3-deleted mutant and its parental strain 70-15

Project description:Genes potentially regulated by the Flb3p transcription factor were identified by microarray-based transcriptome analysis of the flb3- mutant and the parental strain 70-15.

2013-01-01 | GSE38327 | GEO

Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae FLB3-deleted mutant and its parental strain 70-15

Project description:Genes potentially regulated by the Flb3p transcription factor were identified by microarray-based transcriptome analysis of the flb3- mutant and the parental strain 70-15. Two-condition experiment, wild-type vs. FLB3-deleted mutant. 3 biological replicates with dye-swaps

2013-01-01 | E-GEOD-38327 | biostudies-arrayexpress

Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae FLB4-deleted mutant and its parental strain 70-15

Project description:Genes potentially regulated by the Flb4p transcription factor were identified by microarray-based transcriptome analysis of the flb4- mutant and the parental strain 70-15. Two-condition experiment, wild-type vs. FLB4-deleted mutant. 6 biological replicates with dye-swaps

2013-01-01 | E-GEOD-38328 | biostudies-arrayexpress

Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain

2012-03-03 | GSE36224 | GEO

Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain

Project description:In order to understand the function of the Tra1p transcription factor we identified potentially Tra1p-regulated genes by comparison of transcript abundance in aerial mycelium of the tra1- mutant and the parental strain 70-15. Two-condition experiment, wild-type vs. TRA1-deleted mutant. 4 biological replicates with dye-swaps. Each microarry slide had 2 arrays with 44k spots (22k spots printed twice). Thus, rep1 and rep2 are technical replicates of the same biological sample (derived from one raw data file).

2012-03-03 | E-GEOD-36224 | biostudies-arrayexpress

Comparison of transcript abundance in ungerminated spores of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain

2012-03-03 | GSE36225 | GEO

Comparison of transcript abundance in ungerminated spores of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain

2012-03-03 | E-GEOD-36225 | biostudies-arrayexpress

RNA-seq Analysis of MoRFX1-dependent Genes in Magnaporthe oryzae 70-15

Project description:Purpose: The goals of this study are to find out the genes regulated directly or indirectly by the regulatory factor X protein MoRfx1 by RNA-seq in the rice blast fungus Methods: Mycelial mRNAs of the wild type strain 70-15, ΔMorfx1 incubated in H2O at 25˚C for 4 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). RNA-sequencing libraries were constructed using NEBNext RNA sample preparation kit (NEB). Samples were sequenced in a 1 x 100 nt way on Illumina Hiseq2500 instrument using TruSeq PE Cluster Kit v3 - cBot - HS (Illumina) and TruSeq SBS Kit v3-HS (Illumina).The M. oryzae genome database (MG8) (www.broadinstitute.org) was used as a reference gene database. Genome-wide transcript levels of genes were quantified in fragments per kilobase of exon model per million mapped reads (FPKM) (Trapnell 2010). Gene Ontology (GO) enrichment analyses for differentially expressed genes were performed using hypergeometric test with topGO, and p-values were adjusted with Bonferroni for multiple testing (Alexa 2006). And we selected FDR < 0.05 as enrichment terms. Results and Conclusions: We identified 1735 genes regulated by Morfx1 through RNA-seq.

2016-07-20 | GSE65296 | GEO

RNA-seq Analysis of MoBHLH6-dependent Genes in Magnaporthe oryzae 70-15

Project description:Purpose: The goals of this study are to find out the genes regulated directly or indirectly by bHLH protein MoBhlh6 (Crf1) by RNA-seq in the rice blast fungus Methods: Mycelial mRNAs of the wild type strain 70-15, ΔMobhlh6 incubated in H2O at 25˚C for 4 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). RNA-sequencing libraries were constructed using NEBNext RNA sample preparation kit (NEB). Samples were sequenced in a 1 x 100 nt way on Illumina Hiseq2500 instrument using TruSeq PE Cluster Kit v3 - cBot - HS (Illumina) and TruSeq SBS Kit v3-HS (Illumina).The M. oryzae genome database (MG8) (www.broadinstitute.org) was used as a reference gene database. Genome-wide transcript levels of genes were quantified in fragments per kilobase of exon model per million mapped reads (FPKM) (Trapnell 2010). Gene Ontology (GO) enrichment analyses for differentially expressed genes were performed using hypergeometric test with topGO, and p-values were adjusted with Bonferroni for multiple testing (Alexa 2006). And we selected FDR < 0.05 as enrichment terms. Results and Conclusions: We identified 3603 genes regulated by MoBhlh6 through RNA-seq.

2018-08-24 | GSE80679 | GEO

RNA-seq Analysis of MoLID2 (MoKDM5)-dependent Genes in Magnaporthe oryzae 70-15

Project description:Purpose: The goals of this study are to find out the genes regulated directly or indirectly by a trimethyl H3K4 histone demethylase Molid2 (MoKdm5) by RNA-seq in the rice blast fungus Methods: Mycelial mRNAs of the wild type strain 70-15, ΔMolid2 incubated in H2O at 25˚C for 4 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). RNA-sequencing libraries were constructed using NEBNext RNA sample preparation kit (NEB). Samples were sequenced in a 1 x 100 nt way on Illumina Hiseq2500 instrument using TruSeq PE Cluster Kit v3 - cBot - HS (Illumina) and TruSeq SBS Kit v3-HS (Illumina).The M. oryzae genome database (MG8) (www.broadinstitute.org) was used as a reference gene database. Genome-wide transcript levels of genes were quantified in fragments per kilobase of exon model per million mapped reads (FPKM) (Trapnell 2010). Gene Ontology (GO) enrichment analyses for differentially expressed genes were performed using hypergeometric test with topGO, and p-values were adjusted with Bonferroni for multiple testing (Alexa 2006). And we selected FDR < 0.05 as enrichment terms. Results and Conclusions: We identified 3400 genes regulated by Molid2 through RNA-seq.

2021-01-01 | GSE65539 | GEO

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