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Comparison of transcript abundance in ungerminated spores of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain

ABSTRACT: In order to understand the function of the Tra1p transcription factor we identified potentially Tra1p-regulated genes by comparison of transcript abundance in conidia of the tra1- mutant and the parental strain 70-15.

ORGANISM(S): Pyricularia grisea Pyricularia oryzae

PROVIDER: GSE36225 | GEO | 2012/03/03

SECONDARY ACCESSION(S): PRJNA155819

REPOSITORIES: GEO

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Comparison of transcript abundance in ungerminated spores of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain

2012-03-03 | E-GEOD-36225 | biostudies-arrayexpress

Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain

2012-03-03 | GSE36224 | GEO

Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain

Project description:In order to understand the function of the Tra1p transcription factor we identified potentially Tra1p-regulated genes by comparison of transcript abundance in aerial mycelium of the tra1- mutant and the parental strain 70-15. Two-condition experiment, wild-type vs. TRA1-deleted mutant. 4 biological replicates with dye-swaps. Each microarry slide had 2 arrays with 44k spots (22k spots printed twice). Thus, rep1 and rep2 are technical replicates of the same biological sample (derived from one raw data file).

2012-03-03 | E-GEOD-36224 | biostudies-arrayexpress

Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae FLB4-deleted mutant and its parental strain 70-15

Project description:Genes potentially regulated by the Flb4p transcription factor were identified by microarray-based transcriptome analysis of the flb4- mutant and the parental strain 70-15.

2013-01-01 | GSE38328 | GEO

Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae FLB3-deleted mutant and its parental strain 70-15

Project description:Genes potentially regulated by the Flb3p transcription factor were identified by microarray-based transcriptome analysis of the flb3- mutant and the parental strain 70-15.

2013-01-01 | GSE38327 | GEO

Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae FLB3-deleted mutant and its parental strain 70-15

Project description:Genes potentially regulated by the Flb3p transcription factor were identified by microarray-based transcriptome analysis of the flb3- mutant and the parental strain 70-15. Two-condition experiment, wild-type vs. FLB3-deleted mutant. 3 biological replicates with dye-swaps

2013-01-01 | E-GEOD-38327 | biostudies-arrayexpress

Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae FLB4-deleted mutant and its parental strain 70-15

Project description:Genes potentially regulated by the Flb4p transcription factor were identified by microarray-based transcriptome analysis of the flb4- mutant and the parental strain 70-15. Two-condition experiment, wild-type vs. FLB4-deleted mutant. 6 biological replicates with dye-swaps

2013-01-01 | E-GEOD-38328 | biostudies-arrayexpress

Analysis of transcript abundance during appressorium formation in the rice blast fungus Magnaporthe oryzae using HT-SuperSAGE.

Project description:The rice-blast fungus Magnaporthe oryzae is disseminated using a three-celled spore or conidium. Upon landing on the surface of a rice leaf, the conidium germinates and forms a specialised structure for entry into the host plant - the appressorium. In this study we have followed gene expression thoughout germination of the conidium and formation of the appressorium to identify genes that may be important for appressorium function. We have also compared gene expression in the wild-type germinating conidium at four hours to a mutant strain deleted for the MAP kinase pmk1. The pmk1 mutant is unable to form appressoria. Samples were taken from five time-points in wild-type germinating conidia and one time-point in the pmk1 mutant. Two replicates were taken at each time-point.

2012-02-13 | E-GEOD-30069 | biostudies-arrayexpress

Gene expression profile of Tra1 dependent genes

Project description:Promoter-specific transcriptional activators (activators) stimulate transcription through direct interactions with one or more components of the transcription machinery, termed the “target”. Previous studies have provided evidence that the Tra1 subunit of the yeast SAGA (Spt-Ada-Gcn5-acetyltransferase) complex is the target of the yeast activator Gal4. However, several other general transcription factors, in particular the mediator complex, have also been implicated as Gal4 targets. To investigate the essentiality of Tra1 as a target of Gal4, here we derive Tra1 mutants that are selectively defective for interaction with Gal4 in vivo (Gal4 Interaction Defective (GID) mutants). In contrast to wild-type Tra1, Tra1 GID mutants are not recruited by Gal4 to the promoter and cannot support Gal4-directed transcription activation, demonstrating that the Gal4–Tra1 interaction is required for Gal4 function. In yeast strains expressing a Tra1 GID mutant, Gal4 promoter binding is unexpectedly also diminished indicating that Gal4 and Tra1 bind cooperatively. Consistent with cooperative binding, we demonstrate that the interaction between Gal4 and Tra1 occurs predominantly on the promoter and not off DNA. Finally, we show that although Tra1 is also targeted by other activators, these interaction are unaffected by GID mutations, revealing an unanticipated specificity of the Gal4-Tra1 interaction.

2011-12-30 | GSE31390 | GEO

Phosphoproteome Analysis of Magnaporthe oryzae During Appressorium Development

Project description:The rice pathogen, Magnaporthe oryzae, undergoes a complex developmental process leading to formation of an appressorium prior to plant infection. In an effort to better understand phosphoregulation during appressorium development, a mass spectrometry-based study was undertaken. Phosphosites were identified from phosphoproteins from mycelia, conidia, germlings, and appressoria of the wild type and a ckpA mutant. The cyclic-AMP dependent protein kinase A (CPKA) is required for initial perception of the leaf surface, development of functional appressoria, and timely mobilization of storage reserves in conidia, including glycogen and lipid bodies.

2015-05-13 | PXD001389 | Pride

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