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Differentially regulted genes in neural stem cells treated by oleanolic acid

ABSTRACT: Oleanolic acid significantly inhibited neurosphere formation in a dose-dependent manner, and achieved a maximum effect at 10 nM. OA also reduced the 5-ethynyl-2’-deoxyuridine (EdU) incorporation into NSCs, indicating an inhibition on proliferation of NSCs. Next, in western blotting analysis, the protein expression of neuron-specific marker tubulin-βⅢ (TuJ1) and Mash1 was increased, while the astrocyte-specific marker glial fibrillary acidic protein (GFAP) decreased. In immunofluorescence analysis, OA significantly elevated the percentage of TuJ1-positive cells and reduced GFAP-positive cells. Using DNA microarrays, 183 genes were found to be differentially regulated by OA.

ORGANISM(S): Mus musculus

PROVIDER: GSE38394 | GEO | 2012/06/02

SECONDARY ACCESSION(S): PRJNA167824

REPOSITORIES: GEO

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Differentially regulted genes in neural stem cells treated by oleanolic acid

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