Expression Analysis of Normal and Cancerous Prostate Cells
Ontology highlight
ABSTRACT: Strand-specific RNA sequencing was done on a normal and a cancer cell line to examine how isoforms are used differently between these two states.
Project description:Strand-specific RNA sequencing was done on a normal and a cancer cell line to examine how isoforms are used differently between these two states. One PrEC sample, a normal cell line. One LNCaP sample, a cancer cell line.
Project description:Capped analysis of gene expression (CAGE) sequencing was done on a normal and a cancer cell line to examine how promoter usage changes between these two states.
Project description:Capped analysis of gene expression (CAGE) sequencing was done on a normal and a cancer cell line to examine how promoter usage changes between these two states. One PrEC sample, a normal cell line. One LNCaP sample, a cancer cell line.
Project description:H3K4me3 ChIP-seq was done to examine transcription start sites in a genome wide manner, and compare results to CAGE-seq One PrEC sample, a normal cell line. One LNCaP sample, a cancer cell line.
Project description:To investiagate copy number differences between PrEC and LNCaP cells, each was DNA sequenced. One PrEC sample, a normal cell line. One LNCaP sample, a cancer cell line.
Project description:Mapping the locations of the DNA binding protein CTCF genome-wide to assess its colocalisation with expression domain boundaries. One PrEC sample, a normal cell line. One LNCaP sample, a cancer cell line.
Project description:The histone modification H3K27me3 is representative of silenced regions of the genome. Changes between the normal and cancer state were investigated. Three PrEC samples, a normal cell line. Four LNCaP sample, a cancer cell line. Technical replicates.
Project description:To identify aberrant non coding gene expression in prostate cancer, we performed expression profiling of normal prostate epithelial cells (PrEC) and the prostate cancer cell line LNCaP using Affymetrix HuGene 2.0 ST expression arrays. These expression arrays were validated by expression qPCR of selected genes.