Project description:A single-cell assay for transposase-accessible chromatin with high-throughput sequencing (scATAC-seq) was applied to compare the single-cell chromatin accessibility profiles of peripheral blood mononuclear cells (PBMCs) that were obtained from healthy volunteers and uremia patients. Besides, we further revealed the biological functions and regulatory modes of the uremia-related transcription factors (TFs). We principally identified 8 leukocytic clusters using promoter sums of cell type-specific marker genes. Representative TF motifs related to immune cell phenotype identification were found. The comparison of cells from uremia patients and healthy individuals revealed differential candidate TF motifs driving the uremia-associated transcriptomic signature changes, thereby resulting in variation of multiple immune-inflammatory pathways, virus infection-related pathways, and tumor-associated pathways.Our findings reveal transcriptional regulatory variation in uremia-derived PBMCs from an epigenetic perspective, providing a foundational framework for studies examining proinflammatory mechanisms in uremia and establishing a foundation for future research investigating epigenetic therapy.
Project description:Brachylophosaurus canadensis peptides from blood vessels isolated from within the bone were characterized using high resolution mass spectrometry. This allowed identification of actin, alpha and beta tubulin, various histones, myosin, and tropomyosin. Within the peptides, evidence of age (e.g., deamidation, oxidation, protein backbone cleavage) was present.
Project description:Tumor-associated blood vessels differ from normal vessels at the morphological and molecular level. Proteins that are only present on tumor vessels may serve as biomarkers and as therapeutic targets for inhibition of angiogenesis in cancer. Comparing the transcriptional profiles of blood vascular endothelium from human invasive bladder cancer and from normal bladder tissue, we found several markers that could serve as novel biomarkers or therapeutic targets. In this dataset, we include the expression data obtained from laser capture microdissected (LCM) vessels isolated from tumor and bladder normal tissue. 10 samples were analyzed. We compared expression of tumor associated blood vessels with expression of vessels in the normal bladder tissue using Genespring GX 12.
Project description:Tumor-associated blood vessels differ from normal vessels at the morphological and molecular level. Proteins that are only present on tumor vessels may serve as biomarkers and as therapeutic targets for inhibition of angiogenesis in cancer. Comparing the transcriptional profiles of blood vascular endothelium from human invasive bladder cancer and from normal bladder tissue, we found several markers that could serve as novel biomarkers or therapeutic targets. In this dataset, we include the expression data obtained from laser capture microdissected (LCM) vessels isolated from tumor and bladder normal tissue.
Project description:How organ size and form are controlled during development is a major question of biology. Blood vessels have been shown to be essential for early development of the liver and pancreas, and are fundamental to normal and pathological tissue growth. Here we report that non-nutritional signals from blood vessels surprisingly act to restrain pancreas growth. Elimination of endothelial cells increases the size of embryonic pancreatic buds. Conversely, VEGF-induced hypervascularization decreases pancreas size. The growth phenotype results from vascular restriction of pancreatic tip cell formation, lateral branching and differentiation of the pancreatic epithelium into endocrine and acinar cells. The effects are seen both in vivo and ex vivo, indicating a perfusion-independent mechanism. Thus the vasculature controls pancreas morphogenesis and growth by reducing branching and differentiation of primitive epithelial cells.