Dataset Information

Shotgun Proteomics of Brachylophosaurus canadensis Blood Vessels

ABSTRACT: Brachylophosaurus canadensis peptides from blood vessels isolated from within the bone were characterized using high resolution mass spectrometry. This allowed identification of actin, alpha and beta tubulin, various histones, myosin, and tropomyosin. Within the peptides, evidence of age (e.g., deamidation, oxidation, protein backbone cleavage) was present.

INSTRUMENT(S): Fourier Transform Ion Cyclotron Resonance Mass Spectrometer, LTQ Orbitrap XL, LTQ Orbitrap Elite

ORGANISM(S): Gallus Gallus (chicken) Struthio Camelus (ostrich) Brachylophosaurus Canadensis (campanian Hadrosaur)

TISSUE(S): Blood Vessel

SUBMITTER: Leonid Zamdborg

LAB HEAD: Timothy Paul Cleland

PROVIDER: PXD001738 | Pride | 2015-11-30

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Brachy_cid-2_archosaur.mzxml	Mzxml
	Brachy_cid-2_archosaur.pep.xml	Pepxml
	Brachy_cid-2_aves.mzxml	Mzxml
	Brachy_cid-2_aves.pep.xml	Pepxml
	Brachy_cid-2_chicken.mzxml	Mzxml

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Publications

Mass Spectrometry and Antibody-Based Characterization of Blood Vessels from Brachylophosaurus canadensis.

Cleland Timothy P TP Schroeter Elena R ER Zamdborg Leonid L Zheng Wenxia W Lee Ji Eun JE Tran John C JC Bern Marshall M Duncan Michael B MB Lebleu Valerie S VS Ahlf Dorothy R DR Thomas Paul M PM Kalluri Raghu R Kelleher Neil L NL Schweitzer Mary H MH

Journal of proteome research 20151123 12

Structures similar to blood vessels in location, morphology, flexibility, and transparency have been recovered after demineralization of multiple dinosaur cortical bone fragments from multiple specimens, some of which are as old as 80 Ma. These structures were hypothesized to be either endogenous to the bone (i.e., of vascular origin) or the result of biofilm colonizing the empty osteonal network after degradation of original organic components. Here, we test the hypothesis that these structures ...[more]

PMID: 26595531

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