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Expression data from SKOV/ip-Fumock- or SKOV/ip-FuEP2/Ex2-derived tumor

ABSTRACT: We intraperitoneally injected SKOV3 (derived from human ovarian adenocarcinoma) expressing soluble fragment of human EP2 receptor or hIgG Fc (SKOV/ip-FuEP2/Ex2 and (SKOV/ip-Fumock). After 4 week, resulted tumors were lysed and total RNAs were isolated. By using microarray, We analyzed gene expression and identified distinct classes of up-regulated genes in SKOV/ip-FuEP2/Ex2-derived tumor.

ORGANISM(S): Homo sapiens

PROVIDER: GSE38843 | GEO | 2012/06/22

SECONDARY ACCESSION(S): PRJNA169148

REPOSITORIES: GEO

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Expression data from SKOV/ip-Fumock- or SKOV/ip-FuEP2/Ex2-derived tumor

Project description:We intraperitoneally injected SKOV3 (derived from human ovarian adenocarcinoma) expressing soluble fragment of human EP2 receptor or hIgG Fc (SKOV/ip-FuEP2/Ex2 and (SKOV/ip-Fumock). After 4 week, resulted tumors were lysed and total RNAs were isolated. By using microarray, We analyzed gene expression and identified distinct classes of up-regulated genes in SKOV/ip-FuEP2/Ex2-derived tumor. Tumor tissues from SKOV/ip-Fumock or SKOV/ip-FuEP2/Ex2 were lysed and total RNAs were extracted. And then we analyzed expression status by Affymetrix microarrays.

2012-06-21 | E-GEOD-38843 | biostudies-arrayexpress

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Project description:Expression data from SKOV/ip-Fumock- or SKOV/ip-FuEP2/Ex2-derived tumor

| PRJNA169148 | ENA

SKOV-3TR

Project description:Human paclitaxel-resistant (SKOV-3TR) ovarian cancer cell line and the parent SKOV-3 cell line were grown in RPMI medium containing 10% fetal calf serum. The experiment was done to compare the expression differences between the parent SKOV-3 cell line and the derived SKOV-3TR cell line. Cells were plated in T75 flasks. They were cultured until they were 80% confluent and harvested by trypsinization followed by pelleting of the cells. All RNA isolation was accomplished with the Tri-Reagent (SIgma) and purified using the Qiagen RNeasy Mini Kit reagents. The RNA (50 ug) was annealed with a random hexamer primer, and reverse-transcribed into cDNA with Powerscript (Clontech) reverse transcriptase for 2h at 42 degrees in the presence of amino-allyl dUTP. The cDNA from SKOV-3 RNA and SKOV-3TR RNA was covalently coupled separately with Cy3 and Cy5 monoreactive fluors in 50 mM sodium bicarbonate, pH 9.0. The Cy3 and Cy5 labelled cDNAs were combined and purified with a QIAquick PCR purification kit and hybridized to the microarray using the Hybrite chamber (Vysis). Fluorescent array images were collected for both Cy3 and Cy5 with a ScanArray 4000 (Perkin Elmer) at a resolution of 10 um at maximum laser power and photo-multiplier tube voltage of 50 to 60%. Segmentation was performed using the histogram method of the QuantArray (Perkin Elmer) package. Data was transformed into log base 2 expression ratios and normalized using scaled loess normalization. Keywords: repeat sample

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Role of HBO1 (KAT7/MYST2) in ovarian cancer cell line SKOV-3

Project description:Analysis of change in transcriptosome after HBO1 knockdown in SKOV-3 ovarian cancer cell line

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Offect of ATP depletion on SKOV-3 cells using apyrase

Project description:The purpose of this experiment was to identify the effects of ATP depletion (using apyrase) on a metastatic ovarian carcinoma cell line (SKOV-3) and compare it to a non-treated sample. Cells were treated either with media alone or with media with apyrase during 24h. SKOV-3 cells were harvested during 12h prior to incubation. RNA was extracted with TRIZOL reagent and 10ug of RNA were used to synthetize cDNA

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Comparisons of the global gene profile between C-CPE treated- and untreated- SKOV-3 ovarian cancer cells.

Project description:A microarray analysis was performed to compare the global gene expression profile between C-CPE treated- and untreated- SKOV-3 ovarian cancer cells.

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Human SKOV-3 cells, Input DNA vs ChIP DNA

Project description:Identification of target genes of PITX2 homeodomain transcription factor in ovary using human ovarian adenocarcinoma cells, SKOV-3 to determine the transcriptional network of PITX2. Genotypic Technology designed Custom Human Promoter 244k ChIP-on-chip Array (AMADID-019469)

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Project description:MTG1-RNA IP

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