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Leaf transcriptome comparison between wt and urt1 (salk_087647) -Transcriptome comparison between WT and a mutant impaired in RNA uridylation.

ABSTRACT: rs11-10_urt1 - leaf transcriptome comparison between wt and urt1 (salk_087647) - Identification of mRNA targets of the terminal uridylyltransferase URT1 (AT2G45620) in Arabidopsis thaliana. - Identification of the RNA targets of the terminal uridylyl transferase URT1 (AT2G45620). Mature rosettte leaf transcriptomes were compared between WT and a urt1 knock-out mutant (SALK_087647).

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE38902 | GEO | 2012/07/13

SECONDARY ACCESSION(S): PRJNA170487

REPOSITORIES: GEO

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leaf transcriptome comparison between wt and tut1 (salk_087647) -Transcriptome comparison between WT and a mutant impaired in RNA uridylation.

Project description:rs11-10_tut1 - leaf transcriptome comparison between wt and tut1 (salk_087647) - Identification of mRNA targets of the terminal uridylyltransferase TUT1 (AT2G45620) in Arabidopsis thaliana. - Identification of the RNA targets of the terminal uridylyl transferase TUT1 (AT2G45620). Mature rosettte leaf transcriptomes were compared between WT and a tut1 knock-out mutant (SALK_087647). 3 dye-swap - gene knock out

2012-07-12 | E-GEOD-38902 | biostudies-arrayexpress

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The TUTase URT1 connects decapping activators and prevents the accumulation of excessively deadenylated mRNAs to avoid siRNA biogenesis

Project description:Uridylation is a widespread modification destabilizing eukaryotic mRNAs. Yet, molecular mechanisms underlying TUTase-mediated mRNA degradation remain mostly unresolved. Here, we report that the Arabidopsis TUTase URT1 participates in a molecular network connecting several translational repressors/decapping activators. URT1 directly interacts with DECAPPING 5 (DCP5), the Arabidopsis ortholog of human LSM14 and yeast Scd6, and this interaction connects URT1 to additional decay factors like DDX6/Dhh1-like RNA helicases. Nanopore direct RNA sequencing reveals a global role of URT1 in shaping poly(A) tail length, notably by preventing the accumulation of excessively deadenylated mRNAs. Based on in vitro and in planta data, we propose a model that explains how URT1 could reduce the accumulation of oligo(A)-tailed mRNAs both by favoring their degradation and because 3’ terminal uridines intrinsically hinder deadenylation. Importantly, preventing the accumulation of excessively deadenylated mRNAs avoids the biogenesis of illegitimate siRNAs that silence endogenous mRNAs and perturb Arabidopsis growth and development.

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