Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

C.elegans exposed to P. aeurigonsa: Colonized vs Non colonized animals

ABSTRACT: Transcriptional profiling of adult C.elegans exposed to E.coli or to GFP-expressing P. aeruginosa (strain PA14). For P. aeruginosa exposure, worms were separated into 2 groups - fully colonized (green) or non-colonized (dark).

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE39536 | GEO | 2012/07/20

SECONDARY ACCESSION(S): PRJNA171058

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

C.elegans exposed to P. aeurigonsa: Colonized vs Non colonized animals

Project description:Transcriptional profiling of adult C.elegans exposed to E.coli or to GFP-expressing P. aeruginosa (strain PA14). For P. aeruginosa exposure, worms were separated into 2 groups - fully colonized (green) or non-colonized (dark). Three conditions (E.coli, P. aeurginosa colonized, and P. aeurginosa non-colonized). 2-color arrrays, each sample co-hybridized with the same reference RNA sample from mixed stage C.elegans cultures Each condition includes 3 biological replicates - 2 using the wormsorter (WS) to separate colonized from non-colonized and one hand picked (HP) under a fluorescent stereoscope

2012-07-19 | E-GEOD-39536 | biostudies-arrayexpress

C.elegans treated with control or elt-2 RNAi during adulthood and exposed to E. coli or P. aeruginosa

2014-12-04 | GSE63846 | GEO

C.elegans treated with control or elt-2 RNAi during adulthood and exposed to E. coli or P. aeruginosa

Project description:Transcriptional profiling of adult C.elegans exposed to E.coli or to GFP-expressing P. aeruginosa (strain PA14). For P. aeruginosa exposure, worms were separated into 2 groups - fully colonized (green) or non-colonized (dark). Six conditions ( control or elt-2 RNAi ; E.coli, P. aeurginosa colonized, and P. aeurginosa non-colonized). 2-color arrrays, each sample co-hybridized with the same reference RNA sample from mixed stage C.elegans cultures Worms were either sorted using the wormsorter (WS) or were hand picked (HP) under a fluorescent stereoscope

2014-12-04 | E-GEOD-63846 | biostudies-arrayexpress

Gene expression in the small intestine of mice colonized with Pseudomonas aeruginosa

Project description:Celiac disease (CeD) is a food sensitivity characterized by a breakdown of oral tolerance to gluten proteins in genetically predisposed individuals, although the underlying mechanisms are incompletely understood. To evaluate a functional role of bacterial proteases in CeD, we colonized germ-free or clean SPF mice with P.aeruginosa PA14 or P.aeruginosa PA14 disrupted in lasB gene (elastase). We show gluten-independent, PAR-2 mediated upregulation of inflammatory pathways by LasB in C57BL/6 mice without villus blunting. In mice expressing CeD risk genes, P. aeruginosa elastase synergized with gluten to induce more severe inflammation that was associated with moderate villus blunting.

2019-02-01 | GSE125983 | GEO

Time course profiling of IEC and total colonic tissue transcriptome in T.mu colonized animals

Project description:In this experiment we profiled the transcriptome of intestinal colonic epithelium and total colonic tissue of animals colonized with T.mu versus naïve littermate controls at different time points

2017-08-07 | GSE86111 | GEO

P. aeruginosa PA14-WT vs pyrF mutant vs pyrF mutant w/uracil

2008-11-12 | GSE9592 | GEO

Contribution of Veillonella parvula to Pseudomonas aeruginosa mediated pathogenicity in a murine tumor model system

Project description:We investigated the specific interactions of the most dominant bacterial CF-pathogen, Pseudomonas aeruginosa, and the anaerobic bacterium Veilllonella parvula, that has been recovered at comparable cell numbers in the respiratory tract of CF patients. We used our recently established in-vivo murine tumor model to investigate mutual influences of the two pathogens during a biofilm-associated infection process. We found that although P. aeruginosa and V. parvula colonized distinct niches within the tumor, in mice that were co-infected with both bacterial species significant higher cell numbers of P. aeruginosa were recovered from the tumor tissue. Concordantly, in vivo transcriptional profiling implied that the presence of V. parvula supports P. aeruginosa growth at the infected host site, and the higher P. aeruginosa load correlated with clinical deterioration. We cultivated P. aeruginosa PA14 and V. parvula DSM No.:2008 in mono- and co-cultures in vivo using an established murine tumor model. Corresponding in vitro samples were generated under anaerobe growth conditions.

2014-11-06 | E-GEOD-58388 | biostudies-arrayexpress

Expression Data from International C.elegans Experiment 1st

Project description:The effect of microgravity on gene expression in C.elegans was comprehensively analysed by DNA microarray. This is the first DNA microarray analysis for C.elegans grown under microgravity. Hyper gravity and clinorotation experiments were performed as reference against the flight experiment.

2012-03-31 | GSE36358 | GEO

Transcription profiling of P. aeruginosa PA14-WT vs pyrF mutant vs pyrF mutant w/uracil

Project description:Comparative transcriptome analyses of P. aeruginosa PA14 pyrF mutant with PA14 wild type, and with PA14 pyrF mutant with 1 mM uracil supplement and PA14 wild type with 10 mM uracil in biofilm cells. All samples were cultured in LB with glass wool at 37C for 7h. Experiment Overall Design: Strains: P. aeruginosa PA14 wild type, PA14 pyrF mutant Experiment Overall Design: Medium: LB for PA14 wild type and PA14 pyrF mutant, LB with 1 mM uracil for PA14 pyrF mutant, LB with 10 mM uracil for PA14 wild type Experiment Overall Design: Cell type: Biofilm cells grown on glass wool Experiment Overall Design: Time: 7 h Experiment Overall Design: Temperature: 37C

2008-11-14 | E-GEOD-9592 | biostudies-arrayexpress

Within-species control reveals novel immune response genes in Caenorhabditis elegans

Project description:Used P. aeruginosa z11 strain as a control for P. aeruginosa PA14 infection studies in C. elegans

2023-08-31 | GSE205337 | GEO