Transcriptomics

Dataset Information

0

Braveheart is a long non-coding RNA necessary for cardiac lineage commitment


ABSTRACT: Purpose: Long non-coding RNAs (lncRNAs) display development-specific gene expression patterns, yet we know little about their precise roles in lineage commitment. Here, we discover a novel mammalian heart-associated lncRNA, AK143260, necessary for cardiac lineage specification. Methods: Gene expression profiles of mouse ESCs and differentiated organs were analyzed for master regulators of lineage commitment. The AK143260 transcript was shown to be strongly expressed in mESCs and in cells undergoing cardiac differentiation. Its role in cardiac differentiation was examined using depletion and in vitro differentiation systems, with morphological and gene expression profiling at different time-points. Results: mESCs depleted of AK143260, named Braveheart, fail to differentiate into cardiomyocytes and to activate a core cardiac gene regulatory network including key transcription factors driving cardiogenesis. We show that Braveheart functions upstream of MesP1 (mesoderm posterior 1), a transcription factor critical for specification of the earliest known multi-potent cardiovascular progenitor and in promoting epithelial-mesenchymal transition (EMT). Consistent with this, Braveheart depletion leads to morphological defects and loss of cardiogenic potential in a defined in vitro cardiomyocyte differentiation system. Furthermore, Braveheart is necessary to maintain myocardial gene expression and myofibril organization in neonatal cardiomyocytes. Conclusions: These findings reveal that Braveheart is an important regulator of cardiac commitment and implicate lncRNAs as potential therapeutic targets for cardiac disease and regeneration.

ORGANISM(S): Mus musculus

PROVIDER: GSE39656 | GEO | 2013/01/30

SECONDARY ACCESSION(S): PRJNA171341

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2013-01-30 | E-GEOD-39656 | biostudies-arrayexpress
2017-06-22 | GSE64540 | GEO
2019-12-31 | GSE131346 | GEO
2019-12-31 | GSE131337 | GEO
2020-10-23 | GSE159757 | GEO
2018-10-06 | E-MTAB-6268 | biostudies-arrayexpress
2023-06-16 | GSE136692 | GEO
2023-01-20 | GSE157954 | GEO
2023-01-20 | GSE202901 | GEO
2020-10-30 | E-MTAB-7619 | biostudies-arrayexpress