Saccharomyces cerevisiae 3' poly(A) site mapping
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ABSTRACT: The use of alternative polyadenylation sites is common and affects the post-transcriptional fate of mRNA, including its stability, localization, and translation. Here we present a method for genome-wide and strand-specific mapping of poly(A) sites and quantification of RNA levels at unprecedented efficiency by using an on-cluster dark T-fill procedure on the Illumina sequencing platform. Our method outperforms former protocols in quality and throughput, and reveals new insights into polyadenylation in Saccharomyces cerevisiae.
ORGANISM(S): Saccharomyces cerevisiae
PROVIDER: GSE40110 | GEO | 2013/01/10
SECONDARY ACCESSION(S): PRJNA172855
REPOSITORIES: GEO
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