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Transcriptome analysis of Streptococcus mutans biofilms in response to cranberry proanthocyanidins

ABSTRACT: The influence of cranberry proanthocyanidins on the transcriptomic responses of Streptococcus mutans during biofilm formation was investigated.

ORGANISM(S): Streptococcus mutans UA159

PROVIDER: GSE40172 | GEO | 2012/08/22

SECONDARY ACCESSION(S): PRJNA173363

REPOSITORIES: GEO

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Transcriptome analysis of Streptococcus mutans biofilms in response to cranberry proanthocyanidins

Project description:The influence of cranberry proanthocyanidins on the transcriptomic responses of Streptococcus mutans during biofilm formation was investigated. Treatment regimens simulating topical exposures experienced clinically (twice-daily, 60 s each) were used over saliva-coated hydroxyapatite biofilm model. Cranberry proanthocyanidins (1.5 mg/ml) in 15% ethanol was used to treat the biofilms. Four biological replicates each for the treatment and vehicle control were used for RNA extraction and microarray.

2012-08-22 | E-GEOD-40172 | biostudies-arrayexpress

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Streptococcus mutans UA159

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Project description:Transcriptional profiling to investigate the response of Streptococcus mutans biofilms to starch and sucrose. RNA was extracted and purified from four replicate samples of each biofilm sample of interest and labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cells grown to mid-log.

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