Transcriptomics

Dataset Information

0

MRNA profiles following TUT knock-down in HeLa


ABSTRACT: The precise control of microRNA (miRNA) biogenesis is important for various cellular functions, and its dysregulation is often associated with human diseases. We previously reported that Terminal uridylyl transferase 4 (TUT4) down-regulates let-7 miRNA biogenesis by oligo-uridylating let-7 precursor (pre-let-7) in mouse embryonic stem cells and that a pluripotency marker Lin28 promotes a processivity of TUT4. Here we find that TUT4 positively controls let-7 biogenesis by adding a uridine residue to the 3’ end of pre-let-7 in the absence of Lin28. Such mono-uridylation enhances Dicer processing by generating an optimal end structure of pre-let-7 for Dicer recognition and may protect pre-miRNA from trimming. Moreover, TUT7, TUT4 and TUT2 redundantly regulate pre-let-7 processing and simultaneous knock down of these TUTs leads to the decrease of mature let-7 and the accumulation of pre-let-7 in cells. This study provides a novel regulation mechanism of miRNA biogenesis, which may function in development and tumorigenesis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE40228 | GEO | 2012/10/16

SECONDARY ACCESSION(S): PRJNA173241

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2012-10-16 | E-GEOD-40228 | biostudies-arrayexpress
2012-10-16 | E-GEOD-35835 | biostudies-arrayexpress
2012-10-16 | GSE35835 | GEO
2015-04-30 | E-GEOD-64482 | biostudies-arrayexpress
2015-04-30 | GSE64482 | GEO
2022-08-15 | GSE184550 | GEO
2022-08-15 | GSE183384 | GEO
2022-08-15 | GSE203472 | GEO
2020-04-15 | GSE148685 | GEO
2020-04-15 | GSE148686 | GEO