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Retinoic acid determines the in vivo fate-commitment of splenic pre-dendritic cells

ABSTRACT: Comparison of gene expression changes of FACS sorted splenic CD11b+CD8a- and CD11b-CD8a+ cDC subsets reconstituted in vivo following total body irradiation in combination with exogenous retinoic acid or vehicle control.

ORGANISM(S): Mus musculus

PROVIDER: GSE41021 | GEO | 2013/08/16

SECONDARY ACCESSION(S): PRJNA175555

REPOSITORIES: GEO

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Retinoic acid determines the in vivo fate-commitment of splenic pre-dendritic cells

Project description:Comparison of gene expression changes of FACS sorted splenic CD11b+CD8a- and CD11b-CD8a+ cDC subsets reconstituted in vivo following total body irradiation in combination with exogenous retinoic acid or vehicle control. Mice maintained on a control diet were subjected to 6 Gy total body irradiation and then provided exogenous retinoic acid or vehicle control beginning on D(+)3 after irradiation. All treated mice also received adoptive T cell transfer of either Trp-1 CD4+ or Pmel-1 CD8+ T cells, rVV, and IL-2. Splenic cDCs were isolated on D(+)10 after total body irradiation by FACS sorting.

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