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Expression data from E. faecalis Fsr mutants during GBAP induction

ABSTRACT: To rigorously characterize the Fsr regulon, we compared gene expression in V583 isogenic mutants in Fsr genes and each of the Fsr-regulated protease genes using microarrays and purified GBAP, the quorum-sensing molecule. We used microarrays to identified the genes that are regulated directly by Fsr Quorum sensing system.

ORGANISM(S): Enterococcus faecalis Enterococcus faecalis V583

PROVIDER: GSE42036 | GEO | 2013/05/01

SECONDARY ACCESSION(S): PRJNA178964

REPOSITORIES: GEO

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Expression data from E. faecalis Fsr mutants during GBAP induction

Project description:To rigorously characterize the Fsr regulon, we compared gene expression in V583 isogenic mutants in Fsr genes and each of the Fsr-regulated protease genes using microarrays and purified GBAP, the quorum-sensing molecule. We used microarrays to identified the genes that are regulated directly by Fsr Quorum sensing system. E. faecalis V583 cells were collected at 0 minutes and 10 minutes after GBAP addition, for RNA extraction and hybridization on Affymetrix microarrays. The cells were collected when the number of the cells are ideal for Quorum sensing activation. This procedure was also made without GBAP addition. For each condition were made replicates.

2013-05-01 | E-GEOD-42036 | biostudies-arrayexpress

A transcriptome analysis of the Vibrio fischeri LuxR-LuxI regulon

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2011-12-21 | GSE34590 | GEO

Teredinibacter sp. 2052S Quorum Sensing Untargeted Metabolomics

Project description:Raw LC-MS/MS data of crude extract of Teredinibacter sp. 2052S quorum sensing regulon https://gnps.ucsd.edu/ProteoSAFe/status.jsp?task=23f7062e523f49b18e13e4ca13715cfa

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Hierarchical transcriptional regulation of quorum sensing genes in Vibrio harveyi

Project description:Quorum sensing controls hundreds of genes in vibrios required for cell density-specific behaviors, including bioluminescence, biofilm formation, competence, secretion, and motility. The central regulator in the quorum sensing pathway in vibrios is LuxR/HapR, which directly regulates >100 genes in the 625-gene regulon of Vibrio harveyi. Among these directly regulated genes are 15 transcription factors, which we predicted would comprise the second tier in the hierarchy of the quorum sensing regulon. To better study the mechanism of regulation of the quorum sensing network, we mapped all transcriptional start sites in V. harveyi using dRNA-seq. From these data, we determined the relative position of LuxR binding sites in the promoters of genes directly regulated by LuxR. We confirmed that LuxR directly binds to the promoters of the genes encoding transcription factors and quantified the extent of LuxR activation or repression of transcript levels. Finally, we determined the individual regulons for a subset of transcription factors that have not been previously studied. For regulators such as LysR- or AsnC/Lrp-type transcription factors, the regulons contained >100 genes that contained both unique and overlapping genes with the LuxR regulon. These data support a model in which LuxR directly regulates other transcription factors, which act to further alter the second tier of the gene expression cascade producing cell density behaviors in V. harveyi.

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A Distinct QscR Regulon in the Pseudomonas aeruginosa Quorum Sensing Circuit

Project description:To better understand the role of QscR in P. aeruginosa gene regulation and to better understand the relationship between QscR, LasR and RhlR control of gene expression we used transcription profiling to identify a QscR-dependent regulon. Our analysis revealed that QscR activates some genes and represses others. Some of the repressed genes are not regulated by the LasR-I or RhlR-I systems while others are. The LasI-generated 3-oxododecanoyl-homoserine lactone serves as a signal molecule for QscR. Thus QscR appears to be an integral component of the P. aeruginosa quorum sensing circuitry. QscR uses the LasI-generated acyl-homoserine lactone signal and controls a specific regulon that overlaps with the already overlapping LasR and RhlR-dependent regulons. Keywords: Quorum sensing regulon, Direct activation

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mRNA transcriptional profiles in Streptococcus thermophilus wild-type strain LMD-9 and 3 mutants deleted for the pptAB, eep and amiCDE genes.

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Enterococcus faecalis downregulates virulence genes of EHEC

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2016-08-24 | GSE85950 | GEO

The Enterococcus faecalis V583 transcriptional profile during survival within macrophages

2011-12-21 | E-GEOD-34590 | biostudies-arrayexpress

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