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Elimination of mitochondrial DNA variants by nuclear genome transfer in human oocytes.

ABSTRACT: Nuclear genome exchange between different oocytes results in efficient development and stem cell derivation. No differences in homozgyous CNVs were found between swaPS and pES lines. No differences in total copy number variations were found between the swaPS and pES lines.

ORGANISM(S): Homo sapiens

PROVIDER: GSE42271 | GEO | 2013/01/01

SECONDARY ACCESSION(S): PRJNA179512

REPOSITORIES: GEO

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Elimination of mitochondrial DNA variants by nuclear genome transfer in human oocytes.

Project description:Nuclear genome exchange between different oocytes results in efficient development and stem cell derivation. No differences in homozgyous CNVs were found between swaPS and pES lines. No differences in total copy number variations were found between the swaPS and pES lines. DNA was isolated from cultured cells. Affymetrix SNP arrays were used in accordance with the manufacturer's instructions. Copy number analysis was performed using NEXUS 6.0.

2013-01-01 | E-GEOD-42271 | biostudies-arrayexpress

Elimination of mitochondrial DNA variants by nuclear genome transfer in human oocytes

Project description:Nuclear genome exchange between different oocytes results in efficient development and stem cell derivation 1 million cells was used for RNA extraction and labeling using the Illumina total prep RNA amplification kit. Parthenogenetic stem cell lines and embryonic stem cells

2012-12-01 | E-GEOD-42077 | biostudies-arrayexpress

Elimination of mitochondrial DNA variants by nuclear genome transfer in human oocytes

Project description:Nuclear genome exchange between different oocytes results in efficient development and stem cell derivation

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Gene expression in blastomeres after transfer of somatic cells into human oocytes

Project description:The exchange of the oocyte's genome with the genome of a somatic cell, followed by the derivation of pluripotent stem cells, could enable the generation of specific cell types affected in degenerative human diseases. Such cells, carrying the patient's genome, might be useful for cell replacement. Here we report that the development of human oocytes activated after genome exchange invariably arrests at the late cleavage stages in association with transcriptional abnormalities. In contrast, if the oocyte genome is not removed and the somatic cell genome is merely added, they efficiently develop to the blastocyst stage. Human stem cell lines derived from these blastocysts differentiate into cell types of all three germ layers, and a pluripotent gene expression program is established on the genome derived from the somatic cell. This result demonstrates the feasibility of reprogramming human cells using oocytes and identifies the removal of the oocyte genome as the primary cause of developmental failure after genome exchange. Future work should focus on the critical elements that are associated with the human oocyte genome. Somatic cells were transferred into human unfertilized oocytes to determine if human oocytes can reprogram a somatic cell.

2011-10-05 | E-GEOD-28022 | biostudies-arrayexpress

Gene expression in blastomeres after transfer of somatic cells into human oocytes

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Gene expression in pluripotent stem cells derived after somatic cell genome transfer into human oocytes

Project description:The exchange of the oocyte’s genome with the genome of a somatic cell, followed by the derivation of pluripotent stem cells, could enable the generation of specific cell types affected in degenerative human diseases. Such cells, carrying the patient’s genome, might be useful for cell replacement. Here we report that the development of human oocytes activated after genome exchange invariably arrests at the late cleavage stages in association with transcriptional abnormalities. In contrast, if the oocyte genome is not removed and the somatic cell genome is merely added, they efficiently develop to the blastocyst stage. Human stem cell lines derived from these blastocysts differentiate into cell types of all three germ layers, and a pluripotent gene expression program is established on the genome derived from the somatic cell. This result demonstrates the feasibility of reprogramming human cells using oocytes and identifies the removal of the oocyte genome as the primary cause of developmental failure after genome exchange. Future work should focus on the critical elements that are associated with the human oocyte genome. Stem cells were derived by reprogramming of skin cells using oocytes ('nuclear transfer') or defined factors (iPS cells), or from IVF blastocysts

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Project description:Derivation of embryonic stem cells (ESC) genetically identical to a patient by somatic cell nuclear transfer (SCNT) holds the potential to cure or alleviate the symptoms of many degenerative diseases while circumventing any immunorejection issues. However, no primate nuclear transfer embryonic stem (ntES) cell lines have been derived to date. Here, we used a modified SCNT technique to produce rhesus macaque SCNT blastocysts at a relatively high efficiency from adult donor cells and we successfully derived two primate ntES cell lines from 304 oocytes (an overall efficiency of 0.7%). Nuclear and mitochondrial DNA analysis confirmed the ntES cell lines were derived from rhesus monkey SCNT blastocysts and both rhesus monkey ntES cell lines exhibited a normal ESC morphology, expressed key stemness markers, were transcriptionally indistinguishable from control ESC lines and differentiated into multiple cell types. This is, to our knowledge, the first confirmed derivation of primate ntES cell lines. Keywords: rhesus monkey somatic cell nuclear transfer embryonic stem cells

2007-08-29 | GSE7748 | GEO

Gene expression in pluripotent stem cells derived after somatic cell genome transfer into human oocytes

2011-10-06 | GSE27507 | GEO

DNA methylation data from ESCs derived by polar body transfer reconstructed embryos (PBTESCs)

Project description:Here we report the derivation of human ESCs from the polar body thansfer reconstructed embryos. We choose three cell lines from all the cell lines and compare the DNA methylation state. We use human methylation chip to compare genomic DNA methylation level among three PB1 transfer human ES cell lines and three PB2 transfer human ES cell lines.

2018-03-04 | GSE95761 | GEO

Blimp1 expression predicts embryonic stem cell development in vitro

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2011-10-17 | E-GEOD-31868 | biostudies-arrayexpress

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